Globus M, Liversage R
J Embryol Exp Morphol. 1975 Jul;33(4):803-12.
Larval Ambystoma tail regenerates, innervated by the intact spinal cord, were cultured with their epidermal covering in modified Parker's medium (CMRL-1415) and the morphological integrity of the explanted regenerates was maintained with consistent success. The results show that the larval urodele tail blastema is capable of undergoing growth and differentiation when isolated in vitro and regeneration follows the normal in vivo pattern. A diffuse aggregate of blastema cells, which extended caudally from the cut end of the notochord at the time of explantation, underwent differentiation in vitro in the presence of the regenerating spinal cord. The importance of the nerve in regeneration is discussed in relation to the in vitro system.
将有完整脊髓支配的美西螈幼体尾巴及其表皮覆盖物一起，在改良的帕克培养基（CMRL - 1415）中培养，移植的再生组织的形态完整性始终成功得以维持。结果表明，幼体有尾目动物的尾巴芽基在体外分离时能够生长和分化，并且再生遵循正常的体内模式。在移植时从脊索切断端向尾端延伸的一团分散的芽基细胞，在再生脊髓存在的情况下在体外发生分化。结合体外系统讨论了神经在再生中的重要性。
