Globus M, Liversage R A
J Embryol Exp Morphol. 1975 Jul;33(4):813-29.
Explants of 99 adult newt forelimb blastemata (21- to 24-day regenerates) were cultured, with and without implanted dorsal root ganglia, in modified Parker's medium (CMRL-1415) for periods of 72-144 h. Growth and differentiation of the cultured blastemata were compared with ganglionated and non-ganglionated controls fixed at the start of the culture period. The results of these experiments establish that implanted spinal ganglia are able to sustain growth and differentiation of forelimb blastemata in vitro: active proliferation amongst the blastema cells was found to be correlated with the presence of an implanted ganglion. In addition, the blastema cells exhibited a differential growth response which was most pronounced when the ganglion was eccentrically implanted 2-3 days before explantation of the limb regenerate. These results suggest that a causal relationship exists between the position of the implanted ganglion and the localization of growth within the blastema. The nerve influence, believed to be mediated by a chemical factor(s), was localized in the region of the implanted neurons, indicating that a close association between the nerves and the responding blastema cells is essential for normal growth. The importance of the physical presence of nerves for the cultivation of blastemata in vitro is emphasized.
对99个成年蝾螈前肢芽基（21至24天的再生组织）外植体进行培养，在改良的帕克培养基（CMRL - 1415）中，有或没有植入背根神经节，培养72至144小时。将培养的芽基的生长和分化情况与在培养期开始时固定的有神经节和无神经节的对照组进行比较。这些实验结果表明，植入的脊髓神经节能够在体外维持前肢芽基的生长和分化：发现芽基细胞中的活跃增殖与植入神经节的存在相关。此外，芽基细胞表现出不同的生长反应，当在肢体再生组织外植前2至3天偏心植入神经节时，这种反应最为明显。这些结果表明，植入神经节的位置与芽基内生长的定位之间存在因果关系。据信由一种或多种化学因子介导的神经影响定位于植入神经元的区域，这表明神经与反应性芽基细胞之间的紧密关联对于正常生长至关重要。强调了神经的实际存在对于体外培养芽基的重要性。
