[针对尿激酶受体的反义RNA抑制肺癌的侵袭和转移]
[Antisense RNA for urokinase receptor inhibits the invasiveness and metastasis of lung carcinoma].
作者信息
Liao J, Xu S, Tang H, Lian F, Zhu Y
机构信息
Laboratory of Molecular Genetics, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
出版信息
Zhonghua Bing Li Xue Za Zhi. 2001 Oct;30(5):357-60.
OBJECTIVE
To inhibit invasion and metastasis of human lung carcinoma cell line 95D by antisense RNA for urokinase receptor (uPAR).
METHODS
Antisense RNA expression plasmid for uPAR was introduced into the highly metastatic human lung carcinoma cells 95D. Modified Boyden's chamber was used to detect the invasion ability, and nude mice were used to determine metastasis.
RESULTS
Two transfected clones were found to integrate the antisense expression plasmid into genomic DNA and to express antisense RNA for uPAR. Antisense RNA blocked the uPAR expression in the transfected clones. The evident reductions of the invasiveness of antisense clones were observed by comparison with control cells, 95D cells and cells transfected with pcDNA3 vector. The metastatic potential of these two antisense clones decreased significantly in comparison with the controls (P < 0.05).
CONCLUSIONS
The results suggest that antisense RNA for uPAR inhibits uPAR expression and leads to a decrease of metastatic potential of 95D cells. Antisense RNA technique may have a valuable application in anti-metastatic therapy of human lung carcinoma.
目的
通过尿激酶受体（uPAR）反义RNA抑制人肺癌细胞系95D的侵袭和转移。
方法
将uPAR反义RNA表达质粒导入高转移性人肺癌细胞95D。采用改良的Boyden小室检测侵袭能力，并用裸鼠测定转移情况。
结果
发现两个转染克隆将反义表达质粒整合到基因组DNA中，并表达uPAR反义RNA。反义RNA阻断了转染克隆中的uPAR表达。与对照细胞、95D细胞和用pcDNA3载体转染的细胞相比，反义克隆的侵袭性明显降低。与对照相比，这两个反义克隆的转移潜能显著降低（P < 0.05）。
结论
结果表明，uPAR反义RNA抑制uPAR表达并导致95D细胞转移潜能降低。反义RNA技术在人肺癌抗转移治疗中可能具有重要应用价值。
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