Holthues H, Pfeifer-Fukumura U, Hartmann I, Baumann W
Institute of Physical Chemistry, University of Mainz, Germany.
Fresenius J Anal Chem. 2001 Dec;371(7):897-902. doi: 10.1007/s002160101050.
2-Aminobutylamino-4-ethylamino-6-isopropylamino-1,3,5-triazine (ABA-atrazine) has been synthesized and used as a coating hapten in an immunoassay with a monoclonal antibody against terbutryn. Coating was achieved by covalently linking ABA-atrazine to a glutaraldehyde polymer network directly bound to the polystyrene surface of a standard 96-well microtiter plate. The assay was carefully optimized. In particular, the coating hapten concentration had a strong effect on the ELISA sensitivity. By including a pre-incubation step a low test midpoint (IC50-value) of 0.130 microg L(-1) was achieved. As far as we are aware this is the most sensitive ELISA for terbutryn yet reported. The coating-hapten-format presented is proposed as generally applicable, because the glutaraldehyde-modified microtiter plate surface enables stable immobilization of a broad variety of coating haptens.
2-氨基丁基氨基-4-乙基氨基-6-异丙基氨基-1,3,5-三嗪（ABA-莠去津）已被合成，并用作与抗特丁净单克隆抗体免疫分析中的包被半抗原。通过将ABA-莠去津共价连接到直接结合在标准96孔微量滴定板聚苯乙烯表面的戊二醛聚合物网络上来实现包被。该分析方法经过了仔细优化。特别是，包被半抗原浓度对酶联免疫吸附测定（ELISA）灵敏度有很大影响。通过加入预温育步骤，实现了0.130 μg L⁻¹的低检测中点（IC50值）。据我们所知，这是迄今报道的对特丁净最灵敏的ELISA。所呈现的包被半抗原形式被认为具有普遍适用性，因为戊二醛修饰的微量滴定板表面能够稳定固定多种包被半抗原。
