Linder Stefan, Pinkowski Wolfhard, Aepfelbacher Martin
Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Ludwig-Maximilians-Universität, München, Germany.
Biomaterials. 2002 Feb;23(3):767-73. doi: 10.1016/s0142-9612(01)00182-x.
Diamond-like carbon is a promising surface coating for biomedicinal implants like coronary stents or hip joints. Before widespread clinical use of this material, its biocompatibility has to be thoroughly assessed. Cells likely to encounter a diamond-like coated implant in the human body are cells of the monocytic lineage. Their interaction with the diamond-like carbon coated surface will probably critically influence the fate of the implant, as monocytes orchestrate inflammatory reactions and also affect osseointegration of implants. We therefore investigated adhesion, cytoarchitecture and activation status of primary human monocytes and their differentiated derivatives, macrophages, on diamond-like coated glass coverslips using immunofluorescence technique. We show that adhesion of primary monocytes to a diamond-like-coated coverslip is slightly, but not significantly, enhanced in comparison to uncoated coverslips, while the actin and microtubule cytoskeletons of mature macrophages show a normal development. The activation status of macrophages, as judged by polarization of the cell body, was not affected by growth on a diamond-like carbon surface. We conclude that diamond-like carbon shows good indications for biocompatibility to blood monocytes in vitro. It is therefore unlikely that contact with a diamond-like carbon coated surface in the human body will elicit inflammatory signals by these cells.
类金刚石碳是一种很有前景的生物医学植入物表面涂层材料，如冠状动脉支架或髋关节。在这种材料广泛应用于临床之前，必须对其生物相容性进行全面评估。在人体中可能接触到类金刚石涂层植入物的细胞是单核细胞系的细胞。它们与类金刚石碳涂层表面的相互作用可能会对植入物的命运产生关键影响，因为单核细胞会协调炎症反应，还会影响植入物的骨整合。因此，我们使用免疫荧光技术研究了原代人单核细胞及其分化衍生物巨噬细胞在类金刚石涂层玻璃盖玻片上的黏附、细胞结构和激活状态。我们发现，与未涂层的盖玻片相比，原代单核细胞在类金刚石涂层盖玻片上的黏附略有增强，但不显著，而成熟巨噬细胞的肌动蛋白和微管细胞骨架显示出正常发育。通过细胞体极化判断，巨噬细胞的激活状态不受在类金刚石碳表面生长的影响。我们得出结论，类金刚石碳在体外对血液单核细胞显示出良好的生物相容性迹象。因此，在人体中与类金刚石碳涂层表面接触不太可能引发这些细胞产生炎症信号。
