Süss Malte, Washausen Stefan, Kuhn Hans-Jürg, Knabe Wolfgang
Abteilung Morphologie, Zentrum Anatomie der Georg-August-Universität, Kreuzbergring 36, D-37075 Göttingen, Germany.
J Neurosci Methods. 2002 Jan 30;113(2):147-58. doi: 10.1016/s0165-0270(01)00486-1.
Detailed knowledge of the spatial and temporal interactions of distinct cellular events and of the genes involved in their regulation is a precondition for the understanding of morphogenetic and pathogenetic processes. Here, how patterns of cellular events in large objects can be visualized with the help of the image acquisition system 'Huge Image' is demonstrated. Huge images are composed of a multitude of small images scanned with the highest light microscopical resolution. The system is equipped with a programmable autofocus device and permits precise and rapid cytological diagnosis. A vector-based three-dimensional (3-D) reconstruction method which, in future projects, will be combined with 'Huge Image', is applied to visualize dynamic interactions between macrophages and the occurrence of apoptotic neuroepithelial cells in the early developing forebrain of Tupaia belangeri (Scandentia). Proportionally correct meshwire surfaces of small and large objects are generated independently of each other. The combined reconstruction of cellular events and large embryonic surfaces can be carried out from only subsets of histological serial sections, and, compared with volume-based systems, with a much lower need for memory. The practicability of our approach is compared with recent other methods used to demonstrate apoptotic patterns.
详细了解不同细胞事件的时空相互作用以及参与其调控的基因，是理解形态发生和致病过程的前提条件。在此，展示了如何借助图像采集系统“巨型图像”来可视化大型物体中的细胞事件模式。巨型图像由以最高光学显微镜分辨率扫描的大量小图像组成。该系统配备了可编程自动聚焦装置，可进行精确快速的细胞学诊断。一种基于矢量的三维（3-D）重建方法，将在未来项目中与“巨型图像”相结合，用于可视化树鼩（攀鼩目）早期发育前脑巨噬细胞与凋亡神经上皮细胞出现之间的动态相互作用。大小物体的比例正确的网格表面彼此独立生成。细胞事件和大型胚胎表面的联合重建仅可从组织学连续切片的子集中进行，并且与基于体积的系统相比，对内存的需求要低得多。我们的方法的实用性与最近用于展示凋亡模式的其他方法进行了比较。
