Sakamoto M
Jpn J Exp Med. 1975 Jun;45(3):183-9.
The measurement of immune adherence reactivity of rat complement is possible at 20 degrees C but not at 37 degrees C. At 37 degrees C, EA rat C1423b site was destroyed by C3b inactivator present in rat serum. The optimal temperature for immune hemolysis reactivity of rat serum is either at 20 degrees C or 21 degrees C and dose response curve showed Von Krough equation with 1/n = 0.25 +/- 0.02. Al lower temperature, rat complement showed much more effective formation of C3 site to reacti with human erythrocyte in immune adherence as compared with guinea pig complement. The measurement of immune adherence reactivity of rat complement was not possible due to C3b inactivator at 37 degrees C but possible at 20 degrees C. It is possible due to lowering the reactivity of C3b inactivator and keeping the reactivity to form active C3b site in immune adherence reaction. Rat complement is more effective to make C3 site as compared with guinea pig complement. This explains the reason why EDTA rat serum has known to be most effective source of late acting complement for measurement of C1 and C2.
大鼠补体的免疫黏附反应性在20℃时可以检测，但在37℃时不行。在37℃时，EA大鼠C1423b位点被大鼠血清中存在的C3b灭活剂破坏。大鼠血清免疫溶血反应性的最佳温度为20℃或21℃，剂量反应曲线显示符合1/n = 0.25±0.02的冯·克罗伊方程。在较低温度下，与豚鼠补体相比，大鼠补体在免疫黏附中显示出更有效地形成C3位点以与人红细胞反应的能力。由于37℃时存在C3b灭活剂，大鼠补体免疫黏附反应性的检测在该温度下不可行，但在20℃时可行。这是因为降低了C3b灭活剂的反应性，并在免疫黏附反应中保持了形成活性C3b位点的反应性。与豚鼠补体相比，大鼠补体更有效地形成C3位点。这解释了为什么已知EDTA大鼠血清是用于检测C1和C2的最有效的后期补体来源的原因。
