由同源导入系达尔大鼠定义的大鼠2号染色体上的多个血压数量性状位点。

Multiple blood pressure QTL on rat Chromosome 2 defined by congenic Dahl rats.

作者信息

Garrett Michael R, Rapp John P

机构信息

Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo, Ohio 43614-5805, USA.

出版信息

Mamm Genome. 2002 Jan;13(1):41-4. doi: 10.1007/s00335-001-2114-y.

DOI:10.1007/s00335-001-2114-y

PMID:11773968

Abstract

Linkage analysis previously demonstrated a blood pressure quantitative trait locus (QTL) on rat Chromosome 2 (Chr 2) in crosses utilizing Dahl salt-sensitive (S) rats. The present work dissects this QTL by using congenic strains in which segments of Chr 2 from Wistar Kyoto rats (WKY) are placed on the S genetic background. Two distinct QTLs were found where one QTL was anticipated. These each accounted for a blood pressure of 15-20 mm Hg in rats fed 2% NaCl diet for 24 days. One QTL was in the <9-cM interval between D2Rat35 and D2Wox18 (Fgg), and the other was in the <7-cM interval between D2Wox18 (Fgg) and D2Mgh10. A third tentative QTL was suggested, but not clearly established, in the <3-cM interval between D2Mgh10 and D2Rat259.

摘要

连锁分析先前在利用 Dahl 盐敏感（S）大鼠进行的杂交实验中，在大鼠 2 号染色体（Chr 2）上证实了一个血压数量性状基因座（QTL）。目前的工作通过使用近交系来剖析这个 QTL，在这些近交系中，将来自 Wistar Kyoto 大鼠（WKY）的 Chr 2 片段置于 S 遗传背景上。发现了两个不同的 QTL，而原本预计只有一个 QTL。在喂食 2% NaCl 饮食 24 天的大鼠中，这两个 QTL 各自导致血压升高 15 - 20 mmHg。一个 QTL 位于 D2Rat35 和 D2Wox18（Fgg）之间小于 9 cM 的区间内，另一个位于 D2Wox18（Fgg）和 D2Mgh10 之间小于 7 cM 的区间内。在 D2Mgh10 和 D2Rat259 之间小于 3 cM 的区间内，提出了第三个暂定的 QTL，但尚未明确确定。