大鼠9号染色体上血压QTL的连锁分析及同类系构建

Linkage analysis and construction of a congenic strain for a blood pressure QTL on rat chromosome 9.

作者信息

Rapp J P, Garrett M R, Dene H, Meng H, Hoebee B, Lathrop G M

机构信息

Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43614-5804, USA.

出版信息

Genomics. 1998 Jul 15;51(2):191-6. doi: 10.1006/geno.1998.5394.

DOI:10.1006/geno.1998.5394

PMID:9722941

Abstract

A blood pressure quantitative trait locus was found (LOD = 5.0) on rat chromosome 9 using a large F2 population (N = 233) derived from Dahl salt-sensitive (S) and Dahl salt-resistant (R) rats. The F2 rats were fed 8% NaCl diet for 8 weeks. A congenic strain introgressing the R low-blood-pressure QTL allele on chromosome 9 into the S strain was constructed. The congenic strain, designated S.R(chr 9), had a lower blood pressure (19 mm Hg, P < 0.0001) and lower heart weight (112 mg, P < 0.0001) than S rats (2% NaCl diet for 24 days), proving the existence of a blood pressure QTL in the congenic region of about 21 cM.

摘要

利用从达尔盐敏感（S）大鼠和达尔盐抵抗（R）大鼠培育出的一个大型F2群体（N = 233），在大鼠9号染色体上发现了一个血压数量性状位点（LOD = 5.0）。给F2大鼠喂食8% NaCl饮食8周。构建了一个将9号染色体上R低血压QTL等位基因导入S品系的近交系。命名为S.R（chr 9）的近交系大鼠，与S大鼠（24天喂食2% NaCl饮食）相比，血压更低（低19 mmHg，P < 0.0001），心脏重量更轻（轻112 mg，P < 0.0001），这证明在约21 cM的近交区域存在一个血压QTL。