用于诊断小反刍兽疫的病毒分离与聚合酶链反应的比较
Comparison of virus isolation and polymerase chain reaction for diagnosis of peste des petits ruminants.
作者信息
Brindha K, Raj G D, Ganesan P I, Thiagarajan V, Nainar A M, Nachimuthu K
机构信息
Department of Animal Biotechnology, Madras Veterinary College, Chennai, India.
出版信息
Acta Virol. 2001 Jun;45(3):169-72.
Oculonasal swabs and tissue samples collected from peste des petits ruminants (PPR) suspected sheep and goats were tested for presence of the virus of peste des petits ruminants (PPRV) or its RNA by reverse transcription-PCR (RT-PCR) and virus isolation (VI). Of 44 samples 31.8% and 40.9% were positive by VI and RT-PCR, respectively. The RT-PCR-positive samples were subjected to the nested PCR. Three of six samples positive by RT-PCR but negative by VI were negative by the nested PCR. The specificity and accuracy of the nested PCR were higher than those of the RT-PCR although the sensitivity of both tests were similar. Nucleotide sequencing of one nested PCR product revealed a 92% homology with the sequence available in the GenBank (Acc. No. Z37017).
对从小反刍兽疫（PPR）疑似绵羊和山羊采集的眼鼻拭子及组织样本，通过逆转录聚合酶链反应（RT-PCR）和病毒分离（VI）检测小反刍兽疫病毒（PPRV）或其RNA的存在情况。在44份样本中，病毒分离法和RT-PCR法的阳性率分别为31.8%和40.9%。对RT-PCR阳性样本进行巢式PCR。RT-PCR阳性但病毒分离法阴性的6份样本中，有3份经巢式PCR检测为阴性。尽管两种检测方法的敏感性相似，但巢式PCR的特异性和准确性高于RT-PCR。对一份巢式PCR产物进行核苷酸测序，结果显示与GenBank中可获得的序列（登录号Z37017）有92%的同源性。
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