Devi Maitrayee, Das Sutopa, Sharma Krishna, Dutta Rupam
Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022 Assam India.
Department of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122 India.
Virusdisease. 2016 Mar;27(1):91-7. doi: 10.1007/s13337-015-0291-7. Epub 2015 Nov 27.
The present study was undertaken to detect the presence of PPRV in the goats of Assam. Competitive ELISA and Sandwich ELISA are used to detect the PPR viral antibody and antigen respectively. In addition, the study also involved the assessment of specific gene targets for detection of PPRV by RT-PCR from the clinical samples. A total of 579 sera samples (68.65 % in outbreak samples and 5.29 % in random samples) collected from different parts of Assam were tested by c-ELISA, indicated overall prevalence of 27.28 in goats. The percentage prevalence of PPRV antibodies in sera samples from goats collected at the time of outbreaks were 79.26, 85.41, 58.82, 6, 29.41 and 36.36 % in Kamrup, Nalbari, Mongoldoi, Jorhat, Darrang and Barpeta respectively. However, high percent prevalence (20.83 %) was observed in district Dhubri in random samples. Among the suspected samples, high percent prevalence (85.41 %) was observed in Nalbari. The competition percentage values (ranges from 35 to 45) obtained in competitive ELISA from tested goat samples found three categories, viz. positive, doubtful and negative. Most of the serum samples (n = 158) with competition percentage less than or equal to 35 % are considered positive for the presence of PPRV antibodies, (n = 9) greater than 35 % and less than or equal to 45 % are considered doubtful and retested, and (n = 423) greater than 45 % are considered negative. The overall sensitivity, specificity, apparent prevalence and true prevalence rate was found to be 68.65, 94.70, 27.28 and 34.69 % respectively. True prevalence rate was calculated based on the sensitivity and specificity of the c-ELISA employed in the study, which has a relative specificity of 94.70 % and sensitivity of 68.65 %.
本研究旨在检测阿萨姆邦山羊中是否存在小反刍兽疫病毒（PPRV）。分别使用竞争ELISA和夹心ELISA检测PPR病毒抗体和抗原。此外，该研究还涉及通过RT-PCR从临床样本中检测PPRV的特定基因靶点的评估。从阿萨姆邦不同地区收集的总共579份血清样本（暴发样本中占68.65%，随机样本中占5.29%）通过c-ELISA进行检测，结果表明山羊中PPRV的总体流行率为27.28%。在暴发时从山羊采集的血清样本中，PPRV抗体的流行率在卡姆鲁普、纳勒巴里、蒙戈尔多伊、乔哈特、达兰和巴尔佩塔分别为79.26%、85.41%、58.82%、6%、29.41%和36.36%。然而，在随机样本中，杜布里区观察到较高的流行率（20.83%）。在疑似样本中，纳勒巴里观察到较高的流行率（85.41%）。从检测的山羊样本的竞争ELISA中获得的竞争百分比值（范围为35至45）分为三类，即阳性、可疑和阴性。大多数竞争百分比小于或等于35%的血清样本（n = 158）被认为存在PPRV抗体呈阳性，大于35%且小于或等于45%的（n = 9）被认为可疑并重新检测，大于45%的（n = 423）被认为阴性。总体敏感性、特异性、表观流行率和真实流行率分别为68.65%、94.70%、27.28%和34.69%。真实流行率是根据本研究中使用的c-ELISA的敏感性和特异性计算得出的，其相对特异性为94.70%，敏感性为68.65%。
