Malchy B L, Kaplan H
Biochem J. 1976 Oct 1;159(1):173-5. doi: 10.1042/bj1590173.
The reactivity of the amino groups of histones in chromatin towards acetic anhydride was determined as a function of pH. In the pH range 7-10 the vast majority of amino groups in all five histones are buried. However, at higher pH values some of the histone amino groups become exposed, and the higher the lysine:arginine ratio for the histone the greater was the degree of unmasking observed. At pH 11.8 histone I appears to be completely dissociated, histones IIB1 and IIb2 have approx. 55% of the amino groups unmasked, and histones III and IV have approx. 25% of the amino groups unmasked.
测定了染色质中组蛋白氨基对乙酸酐的反应活性与pH值的关系。在pH值7 - 10范围内，所有五种组蛋白中的绝大多数氨基都被掩盖。然而，在较高的pH值下，一些组蛋白氨基会暴露出来，并且组蛋白的赖氨酸:精氨酸比值越高，观察到的暴露程度就越大。在pH 11.8时，组蛋白I似乎完全解离，组蛋白IIB1和IIb2约有55%的氨基暴露，组蛋白III和IV约有25%的氨基暴露。
