Chen Y, Wu Q, Chen Z, Chen F, Su W
174th Hospital of PLA, Xiamen 361005, China.
Chin Med J (Engl). 2000 Apr;113(4):345-9.
To study the effect of all-trans retinoic acid on growth of xenograft tumor and its metastasis in nude mice.
Human gastric cancer BGC-823 and MKN-45 cells were inoculated into spleen subcapsule of nude mice, respectively. The nude mice were subsequently administered with all-trans retinoic acid every other day. Food consuming and body weight of nude mice were measured weekly. Six weeks later, the nude mice were killed. Xenograft tumors in spleen and metastatic tumors in liver were pathologically examined. Microvessel density in the tumors was detected immunohistochemically, and serum carcinoembryonic antigen was measured by radioimmunoassay.
After the nude mice were fed with all-trans retinoic acid, the growth of splenic tumor and its liver metastasis were inhibited and the metastatic rates decreased by 50% (BGC-823) and 33.3% (MKN-45), respectively. The microvessel density in splenic and hepatic tumors reduced by 28.58% and 35.47% (BGC-823), 19.45% and 14.52% (MKN-45), respectively. The concentration of carcinoembryonic antigen decreased by 50.24% (BGC-823) and 48.10% (MKN-45).
All-trans retinoic acid may effectively inhibit the growth of xenograft tumor in spleen and its metastasis to liver in nude mice, which can be corroborated by the decrease of carcinoembryonic antigen and microvessel density.
研究全反式维甲酸对裸鼠移植瘤生长及其转移的影响。
将人胃癌BGC - 823和MKN - 45细胞分别接种于裸鼠脾脏被膜下。随后每隔一天给裸鼠施用全反式维甲酸。每周测量裸鼠的食物消耗量和体重。六周后，处死裸鼠。对脾脏中的移植瘤和肝脏中的转移瘤进行病理检查。免疫组织化学检测肿瘤中的微血管密度，放射免疫法测定血清癌胚抗原。
给裸鼠喂食全反式维甲酸后，脾脏肿瘤的生长及其肝转移受到抑制，转移率分别降低了50%（BGC - 823）和33.3%（MKN - 45）。脾脏和肝脏肿瘤中的微血管密度分别降低了28.58%和35.47%（BGC - 823），19.45%和14.52%（MKN - 45）。癌胚抗原浓度分别降低了50.24%（BGC - 823）和48.10%（MKN - 45）。
全反式维甲酸可有效抑制裸鼠脾脏移植瘤的生长及其向肝脏的转移，癌胚抗原和微血管密度的降低可证实这一点。
