用日本血吸虫抗独特型单克隆抗体NP30免疫小鼠的抗生育免疫
Anti-fecundity immunity in mice immunized with anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum.
作者信息
Feng Z, Li Y, Qiu Z, Li Y, Xue W, Guan X, Wu G
机构信息
Department of Parasitology, Nanjing Medical University, Nanjing 210029, China.
出版信息
Chin Med J (Engl). 2000 Mar;113(3):265-8.
OBJECTIVE
To observe the effects of anti-fecundity and anti-embryonation immunity of anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum on female adult worm.
METHODS
The active immunization of C57BL/6 mice was conducted by means of three intraperitoneal injections of NP30. The control group was injected with SP2/0 ascites intraperitoneally.
RESULTS
On the twenty-seventh day after challenge infection, the number of eggs in the liver tissue and in uterus of the group immunized with NP30 decreased by 30.91% and by 38.55%, respectively. On the thirty-ninth day after the challenge infection, the number of mature eggs in the liver tissue of the group immunized with NP30 decreased by 66.63% and the number of dead eggs increased by 60.66%.
CONCLUSIONS
NP30, with which mice were actively immunized, possesses double effects of anti-fecundity and anti-embryonation immunity on female adult worm of Schistosoma japonicum, therefore it can be used as a promising candidate of anti-pathologic vaccine molecule against Schistosomiasis japonica.
目的
观察日本血吸虫抗独特型单克隆抗体NP30的抗生育和抗胚免疫对日本血吸虫雌虫的作用。
方法
通过腹腔注射NP30对C57BL/6小鼠进行主动免疫。对照组腹腔注射SP2/0腹水。
结果
攻击感染后第27天，NP30免疫组肝脏组织和子宫内虫卵数分别减少30.91%和38.55%。攻击感染后第39天，NP30免疫组肝脏组织中成熟虫卵数减少66.63%，死卵数增加60.66%。
结论
主动免疫小鼠的NP30对日本血吸虫雌虫具有抗生育和抗胚免疫双重作用，因此可作为抗日本血吸虫病病理疫苗分子的有前途的候选物。
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