实验性自身免疫性葡萄膜视网膜炎中浸润细胞的凋亡
Apoptosis of infiltrating cells in experimental autoimmune uveoretinitis.
作者信息
Yang P, Herzberg N H, Zhou H, Broersma L, de Smet M, Kijlstra A
机构信息
Key Laboratory of Health Ministry of China, Zhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Science, Guangzhou 510060, China.
出版信息
Chin Med J (Engl). 2000 Jul;113(7):643-6.
OBJECTIVE
To investigate the cellular phenotype and apoptosis of infiltrating cells involved in experimental autoimmune uveoretinitis (EAU).
METHODS
Immunohistochemical staining and in situ apoptosis staining were performed using monoclonal antibodies to monocytes and macrophages (ED1), major histocompatibility complex (MHC) class-II antigen (OX6), T lymphocytes (R73) and TACS 1 Klenow kit on both ocular sections and wholemounts from Lewis rats after immunization with interphotoreceptor retinoid-binding protein (IRBP).
RESULTS
EAU was induced in 12 of 16 Lewis rats with a mean clinical inflammation score of 1.29 +/- 0.7. Influx of monocytes, lymphocytes and MHC class II-positive cells into the uvea and retina was noted after immunization with IRBP. Apoptosis of infiltrating cells was observed in the uvea and retina, and more apoptotic cells were present in the iris and ciliary body compared with those in the choroid and retina.
CONCLUSION
Apoptosis of infiltrating cells occurs at the early stage of EAU, which may greatly contribute to the rapid regression of the inflammation induced by IRBP.
目的
研究实验性自身免疫性葡萄膜视网膜炎（EAU）中浸润细胞的细胞表型和凋亡情况。
方法
在用光感受器间维生素A结合蛋白（IRBP）免疫Lewis大鼠后，使用针对单核细胞和巨噬细胞（ED1）、主要组织相容性复合体（MHC）II类抗原（OX6）、T淋巴细胞（R73）的单克隆抗体以及TACS 1 Klenow试剂盒，对眼部切片和整装标本进行免疫组织化学染色和原位凋亡染色。
结果
16只Lewis大鼠中有12只诱发了EAU，平均临床炎症评分为1.29±0.7。在用IRBP免疫后，观察到单核细胞、淋巴细胞和MHC II类阳性细胞流入葡萄膜和视网膜。在葡萄膜和视网膜中观察到浸润细胞的凋亡，与脉络膜和视网膜相比，虹膜和睫状体中的凋亡细胞更多。
结论
浸润细胞的凋亡发生在EAU的早期阶段，这可能极大地促使由IRBP诱导的炎症迅速消退。
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