Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats.

OBJECTIVE

To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats.

METHODS

Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used.

RESULTS

Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group.

CONCLUSION

Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.