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伊洛前列素可下调黏附分子的表达,并减轻腹主动脉缺血再灌注所致的肾损伤。

Iloprost downregulates expression of adhesion molecules and reduces renal injury induced by abdominal aortic ischemia-reperfusion.

作者信息

Kiris Ilker, Tekin Ilker, Yilmaz Nigar, Sutcu Recep, Karahan Nermin, Ocal Ahmet

机构信息

Department of Cardiovascular Surgery, Suleyman Demirel University Medical School, Isparta, Turkey.

出版信息

Ann Vasc Surg. 2009 Mar;23(2):212-23. doi: 10.1016/j.avsg.2008.06.010. Epub 2008 Sep 6.

Abstract

The aim of this study was to examine the effect of iloprost in renal injury induced by abdominal aortic ischemia-reperfusion (IR) and how it can modulate the expression of adhesion molecules during this effect. Twenty-four Wistar-Albino rats were randomized into three groups (n=8) as follows: control (sham laparotomy), aortic IR (120 min ischemia and 120 min reperfusion), and aortic IR + iloprost (0.45 microg/kg/hr intravenous infusion during 120 min reperfusion). Blood and renal tissue samples were obtained for biochemical analysis. A histological evaluation with both hematoxylin-eosin staining and immunostaining was also done. Biochemical analyses showed that aortic IR significantly increased (p<0.05 vs. control) whereas iloprost significantly decreased (p<0.05 vs. aortic IR) plasma levels of malondialdehyde, P-selectin, intercellular adhesion molecule-1 (ICAM-I), and tissue levels of malondialdehyde and catalase. Histological evaluation with immunostaining showed that aortic IR significantly increased (p<0.05 vs. control) whereas iloprost significantly decreased (p<0.05 vs. aortic IR) the immunoreactivity of P-selectin, tumor necrosis factor-alpha, CD11b, CD18, and ICAM-1. Hematoxylin-eosin staining showed that iloprost also attenuated the morphological changes associated with aortic IR. The results of this study show that iloprost reduces renal injury induced by aortic IR in rats and downregulates expression of adhesion molecules at both the local and systemic levels after aortic IR during this protective effect.

摘要

本研究的目的是探讨伊洛前列素对腹主动脉缺血再灌注(IR)诱导的肾损伤的影响,以及在这一过程中它如何调节黏附分子的表达。将24只Wistar-白化大鼠随机分为三组(n = 8),如下:对照组(假手术)、主动脉IR组(缺血120分钟和再灌注120分钟)、主动脉IR +伊洛前列素组(再灌注120分钟期间静脉输注0.45微克/千克/小时)。采集血液和肾组织样本进行生化分析。还进行了苏木精-伊红染色和免疫染色的组织学评估。生化分析表明,主动脉IR显著升高(与对照组相比,p < 0.05),而伊洛前列素显著降低(与主动脉IR组相比,p < 0.05)丙二醛、P-选择素、细胞间黏附分子-1(ICAM-1)的血浆水平以及丙二醛和过氧化氢酶的组织水平。免疫染色的组织学评估表明,主动脉IR显著升高(与对照组相比,p < 0.05),而伊洛前列素显著降低(与主动脉IR组相比,p < 0.05)P-选择素、肿瘤坏死因子-α、CD11b、CD18和ICAM-1的免疫反应性。苏木精-伊红染色表明,伊洛前列素还减轻了与主动脉IR相关的形态学变化。本研究结果表明,伊洛前列素可减轻大鼠主动脉IR诱导的肾损伤,并在这一保护作用期间下调主动脉IR后局部和全身水平的黏附分子表达。

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