Meng X, Cheng G, Wang L
Shanghai Hematology Research Institute, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhonghua Zhong Liu Za Zhi. 2000 Jan;22(1):22-5.
In order to investigate the leukemogenic potential of PLZF-RARA fusion protein in vivo, hCG-PLZF-RARA transgenic mice were generated, in which PIZF-RARA fusion gene was driven by hCG promoter to express in myeloid cells of mice.
Molecular cloning technology was used to construct hCG-PLZF-RARA gene. The genotype and phenotype of the hCG-PLZF-RARA transgenic mice were analyzed by PCR, RT-PCR, immunofluorescence, morphology of bone marrow (BM) cells, pathology and retinoic acid differentiation assays.
Six hCG-PLZF-RARA transgenic mice developed leukemia resembling human chronic myeloid leukemia. TF(tissue factor) was not expressed in BM cells of normal mice nor in mice without the expressed transgene, but it was expressed in mice expessing the transgene.
PLZF-RARA fusion protein plays a crucial role in leukemogenesis. TF is up-regulated by PLZF-RARA fusion gene.
为了在体内研究PLZF-RARA融合蛋白的致白血病潜能，构建了hCG-PLZF-RARA转基因小鼠，其中PLZF-RARA融合基因由hCG启动子驱动在小鼠髓系细胞中表达。
采用分子克隆技术构建hCG-PLZF-RARA基因。通过PCR、RT-PCR、免疫荧光、骨髓细胞形态学、病理学及视黄酸分化试验分析hCG-PLZF-RARA转基因小鼠的基因型和表型。
6只hCG-PLZF-RARA转基因小鼠发生了类似于人类慢性髓性白血病的白血病。组织因子（TF）在正常小鼠的骨髓细胞中不表达，在未表达转基因的小鼠中也不表达，但在表达转基因的小鼠中表达。
PLZF-RARA融合蛋白在白血病发生中起关键作用。TF由PLZF-RARA融合基因上调表达。
