Xing B, Ren Z, Su C
Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730.
Zhonghua Zhong Liu Za Zhi. 1999 Jan;21(1):13-5.
To study IL-4 gene therapy for glioma.
Using recombinant retrovirus vector, mIL-4 was transfected into psi 2 packaging cells and rat C6 glioma cells, respectively by lipofectamine. The IL-4 secretting cell clones psi 2 IL-4 and C6IL-4 were studied in vitro and in vivo.
Apart from a decrease in cloning efficiency, no difference was observed between the transfected and the wild type glioma cells in proliferation and cell cycle in vitro. Tumorigenicity of subcutaneously and intracranially inoculated C6IL-4 was reduced. Tumor growth could be suppressed by perineoplastic inoculation of psi 2 IL-4 cells.
IL-4 gene-modified C6 glioma has decreased tumorigenicity. Local production of IL-4 by psi 2 IL-4 cells can lead to regression of established glioma.
研究白细胞介素-4（IL-4）基因治疗胶质瘤。
利用重组逆转录病毒载体，通过脂质体分别将小鼠IL-4（mIL-4）转染至psi 2包装细胞和大鼠C6胶质瘤细胞。对分泌IL-4的细胞克隆psi 2 IL-4和C6IL-4进行体内外研究。
体外实验中，除克隆效率降低外，转染的胶质瘤细胞与野生型胶质瘤细胞在增殖和细胞周期方面未观察到差异。皮下和颅内接种的C6IL-4致瘤性降低。瘤周接种psi 2 IL-4细胞可抑制肿瘤生长。
IL-4基因修饰的C6胶质瘤致瘤性降低。psi 2 IL-4细胞局部产生IL-4可使已形成的胶质瘤消退。
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