[白细胞介素-24基因表达对胶质瘤细胞体外及体内生长的影响]
[Effect of IL-24 gene expression on the growth of glioma cells in vitro and in vivo].
作者信息
Li Wen-ling, Shan Bao-en, Yan Yun-li, Zheng Li-fen, Li Qiao-xia, Zhou Na-jing, Zhao Wen-qing
机构信息
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2005 Mar;27(3):141-4.
OBJECTIVE
To investigate the effect of IL-24 expression on the growth of glioma cells.
METHODS
The IL-24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 glioma cells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor.
RESULTS
It was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell.
CONCLUSION
IL-24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.
目的
研究白细胞介素-24（IL-24）表达对胶质瘤细胞生长的影响。
方法
用逆转录病毒载体将IL-24基因转染至大鼠胶质瘤C6细胞。通过逆转录聚合酶链反应（RT-PCR）证实C6/IL-24胶质瘤细胞中IL-24的表达。采用噻唑蓝（MTT）比色法和流式细胞术研究体外肿瘤细胞增殖。通过脑内接种肿瘤的生长情况，在近交系SD雄性大鼠体内研究其致瘤性。
结果
RT-PCR证实外源性IL-24基因在C6/IL-24细胞中表达。与亲代C6细胞相比，C6/IL-24细胞的体外增殖及体内致瘤性均受到抑制。
结论
胶质瘤细胞中IL-24的表达在一定程度上抑制其体内外生长。
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