[谷胱甘肽S-转移酶π基因在人食管癌发生中的表达]

[Expression of GST-pi gene in human esophageal carcinogenesis].

作者信息

Fu B, Zhang Y, Wang Y

机构信息

Department of Pathology, First Affiliated Hospital, Henan Medical University, Henan Key Laboratory of Tumor Pathology, Zhengzhou 450052.

出版信息

Zhonghua Zhong Liu Za Zhi. 1999 Jan;21(1):29-31.

PMID:11776791

Abstract

OBJECTIVE

To investigate the possible role of GST-pi in esophageal carcinogenesis.

METHODS

GST-pi expression at mRNA level was studied by in situ hybridization (ISH) and at protein level by immunohistochemistry (IHC). GST-pi expression of the esophagus in normal epithelial cells (NC), hyperplastic cells (HC), dysplastic cells (DC) from grade I to III, carcinoma in-situ (CIS) and invasive carcinoma (IC) was examined in the same esophageal cancer specimens (n = 48) which provided a model reflecting the process of esophageal carcinogenesis.

RESULTS

The positive rate of IHC staining was 87.5% for NC, 95.3% for HC, 55.9% for DC (grade I: 73.9%, grade II: 47.4%, grade III: 41.2%), 36.4% for CIS and 45.8% for IC. The positive rate of GST-pi mRNA expression was 81.2% for NC, 94.4% for HC, 61.9% for DC (grade I: 76.5%, grade II: 61.5%, grade III: 41.7%), 44.4% for CIS and 83.3% for grade I IC, 30.0% for grade II IC and 0% for grade III IC. There was no statistically significant difference in GST-pi expression at the mRNA and the protein level.

CONCLUSION

There is a decreasing tendency of GST-pi expression from dysplasia to CIS and IC. The decrease in GST-pi expression is an early event in esophageal carcinogenesis.

摘要

目的

探讨谷胱甘肽 S-转移酶π（GST-pi）在食管癌发生中的可能作用。

方法

采用原位杂交（ISH）技术研究 GST-pi 在 mRNA 水平的表达，采用免疫组织化学（IHC）技术研究其在蛋白质水平的表达。在同一组食管癌标本（n = 48）中检测正常上皮细胞（NC）、增生细胞（HC）、Ⅰ至Ⅲ级发育异常细胞（DC）、原位癌（CIS）和浸润癌（IC）中食管的 GST-pi 表达，该组标本提供了一个反映食管癌发生过程的模型。

结果

免疫组织化学染色阳性率在 NC 中为 87.5%，HC 中为 95.3%，DC 中为 55.9%（Ⅰ级：73.9%，Ⅱ级：47.4%，Ⅲ级：41.2%），CIS 中为 36.4%，IC 中为 45.8%。GST-pi mRNA 表达阳性率在 NC 中为 81.2%，HC 中为 94.4%，DC 中为 61.9%（Ⅰ级：76.5%，Ⅱ级：61.5%，Ⅲ级：41.7%），CIS 中为 44.4%，Ⅰ级 IC 中为 83.3%，Ⅱ级 IC 中为 30.0%，Ⅲ级 IC 中为 0%。GST-pi 在 mRNA 和蛋白质水平的表达无统计学显著差异。