May Karen M, Reynolds Nicola, Cullen C Fiona, Yanagida Mitsuhiro, Ohkura Hiroyuki
The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK.
J Cell Biol. 2002 Jan 7;156(1):23-8. doi: 10.1083/jcb.200106150. Epub 2002 Jan 3.
The fission yeast plo1(+) gene encodes a polo-like kinase, a member of a conserved family of kinases which play multiple roles during the cell cycle. We show that Plo1 kinase physically interacts with the anaphase-promoting complex (APC)/cyclosome through the noncatalytic domain of Plo1 and the tetratricopeptide repeat domain of the subunit, Cut23. A new cut23 mutation, which specifically disrupts the interaction with Plo1, results in a metaphase arrest. This arrest can be rescued by high expression of Plo1 kinase. We suggest that this physical interaction is crucial for mitotic progression by targeting polo kinase activity toward the APC.
裂殖酵母plo1(+)基因编码一种polo样激酶，它是保守激酶家族的成员之一，在细胞周期中发挥多种作用。我们发现，Plo1激酶通过Plo1的非催化结构域和亚基Cut23的四肽重复结构域与后期促进复合物（APC）/细胞周期体发生物理相互作用。一个新的cut23突变特异性地破坏了与Plo1的相互作用，导致中期停滞。这种停滞可以通过Plo1激酶的高表达来挽救。我们认为，这种物理相互作用通过将polo激酶活性靶向APC，对有丝分裂进程至关重要。
