人类-黑猩猩比较克隆图谱的构建与分析。

Construction and analysis of a human-chimpanzee comparative clone map.

作者信息

Fujiyama Asao, Watanabe Hidemi, Toyoda Atsushi, Taylor Todd D, Itoh Takehiko, Tsai Shih-Feng, Park Hong-Seog, Yaspo Marie-Laure, Lehrach Hans, Chen Zhu, Fu Gang, Saitou Naruya, Osoegawa Kazutoyo, de Jong Pieter J, Suto Yumiko, Hattori Masahira, Sakaki Yoshiyuki

机构信息

RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

出版信息

Science. 2002 Jan 4;295(5552):131-4. doi: 10.1126/science.1065199.

DOI:10.1126/science.1065199

PMID:11778049

Abstract

The recently released human genome sequences provide us with reference data to conduct comparative genomic research on primates, which will be important to understand what genetic information makes us human. Here we present a first-generation human-chimpanzee comparative genome map and its initial analysis. The map was constructed through paired alignment of 77,461 chimpanzee bacterial artificial chromosome end sequences with publicly available human genome sequences. We detected candidate positions, including two clusters on human chromosome 21 that suggest large, nonrandom regions of difference between the two genomes.

摘要

最近公布的人类基因组序列为我们提供了参考数据，以便对灵长类动物进行比较基因组研究，这对于理解哪些遗传信息造就了人类至关重要。在此，我们展示了第一代人类-黑猩猩比较基因组图谱及其初步分析。该图谱是通过将77461个黑猩猩细菌人工染色体末端序列与公开的人类基因组序列进行配对比对构建而成的。我们检测到了候选位置，包括人类21号染色体上的两个簇，这表明两个基因组之间存在大片非随机差异区域。