Abiko Y, Nishimura M, Rahemtulla F, Mizoguchi I, Kaku T
Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido, Japan.
Eur J Morphol. 2001 Apr;39(2):99-104. doi: 10.1076/ejom.39.2.99.7372.
In this study, we report the immunohistochemical localization of versican in healthy porcine gingival epithelia. The monoclonal antibody (mAb), 5D5, specifically recognizes core proteins of large chondroitin sulphate proteoglycans such as versican, neurocan and brevican, but not the core protein of aggrecan. Because neurocan and brevican appear to be specific to nervous tissue, the large chondroitin sulphate proteoglycans examined in this study is most likely versican. In the keratinized layer of the attached gingival epithelium, the basal and spinous cell surfaces showed intense staining for mAb 5D5. In the parakeratinized layer of the sulcus epithelium, the localization was restricted to the basal and lower spinous layers. In the junctional epithelium, intense staining was observed in one or two cell layers near the enamel surface. Immunoelectron microscopy revealed high-density depositions of 5D5 immunoreactivity on epithelial cell surfaces. At the enamel surface, 5D5 immunoreactivity was localized to the dental cuticle of the junctional epithelium but was not present in the internal basal lamina. These results suggest that versican, a large chondroitin sulphate proteoglycan, is involved in epithelial differentiation and downgrowth.
在本研究中，我们报告了多功能蛋白聚糖在健康猪牙龈上皮中的免疫组织化学定位。单克隆抗体（mAb）5D5能特异性识别大型硫酸软骨素蛋白聚糖的核心蛋白，如多功能蛋白聚糖、神经蛋白聚糖和短蛋白聚糖，但不能识别聚集蛋白聚糖的核心蛋白。由于神经蛋白聚糖和短蛋白聚糖似乎是神经组织特有的，因此本研究中检测的大型硫酸软骨素蛋白聚糖很可能是多功能蛋白聚糖。在附着龈上皮的角化层，基底细胞和棘细胞表面对mAb 5D5呈强染色。在龈沟上皮的不全角化层，其定位局限于基底和下棘层。在结合上皮中，在靠近釉质表面的一或两层细胞中观察到强染色。免疫电子显微镜显示5D5免疫反应性在上皮细胞表面有高密度沉积。在釉质表面，5D5免疫反应性定位于结合上皮的牙小皮，但在内部基底层不存在。这些结果表明，大型硫酸软骨素蛋白聚糖多功能蛋白聚糖参与上皮分化和向下生长。
