Ng T B, Lam Y W
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, People's Republic of China.
Biochem Biophys Res Commun. 2002 Jan 11;290(1):563-8. doi: 10.1006/bbrc.2001.6235.
A hemagglutinin, with a molecular weight of 30,000 and expressing hemagglutinating activity which could not be inhibited by simple sugars and glycoproteins, was isolated from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji. The protein was adsorbed on CM-Sepharose even in 20 mM ammonium acetate (pH 5.5) containing 1 M NaCl and was desorbed by 20 mM ammonium bicarbonate (pH 9). The hemagglutinating activity was subsequently adsorbed on Mono S in 20 mM ammonium acetate (pH 5.5) and was desorbed by a linear gradient of 0.2-0.5 M NaCl in ammonium acetate buffer. The hemagglutinin exhibited a novel N-terminal sequence not found in any lectin and hemagglutinin reported so far. It was devoid of antifungal activity.
从食用蘑菇姬松茸的新鲜子实体中分离出一种血凝素，其分子量为30,000，具有血凝活性，且该活性不能被单糖和糖蛋白抑制。即使在含有1 M NaCl的20 mM醋酸铵（pH 5.5）中，该蛋白也能吸附在CM-琼脂糖上，并能用20 mM碳酸氢铵（pH 9）解吸。随后，血凝活性在20 mM醋酸铵（pH 5.5）中吸附到Mono S上，并用醋酸铵缓冲液中0.2 - 0.5 M NaCl的线性梯度解吸。该血凝素呈现出一种迄今为止在任何已报道的凝集素和血凝素中均未发现的新的N端序列。它没有抗真菌活性。
