人壶腹周围肿瘤9号染色体区域9p21的详细缺失图谱分析。

Detailed deletion mapping on chromosome region 9p21 in human periampullary neoplasms.

作者信息

Wang C, Lu X, Liu G, Dai L, Xu T, Chen Y, Gao C, Wen X, Qian J

机构信息

Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China.

出版信息

Chin Med J (Engl). 2001 Jun;114(6):588-91.

PMID:11780432

Abstract

OBJECTIVE

To further define the extent of chromosome 9p21 deletion in periampullary neoplasms.

METHODS

The loss of heterozygosity at 5 microsatellite polymorphic markers on chromosome 9p21 was detected by polymerase chain reaction (PCR), polyacrylamide gel electrophoresis (PAGE) and silver staining in 35 specimens of periampullary neoplasms and their matching blood samples.

RESULTS

Fifty percent (4/8) of pancreatic cancer cases showed the loss of heterozygosity at one or more microsatellite loci, with the more frequent sites of D9S974 (37.5%) and D9S942 (28.6%), and some showing consecutive allelic loss. Sixty-two point five percent (5/8) of ampullary carcinoma cases showed loss of heterozygosity at one or more of the loci, frequent site of loss being D9S942 (42.9%) and the next most frequent being IFNA (37.5%) and D9S171 (37.5%). Loss of one locus was observed in 14.2% (1/7) of insulinoma.

CONCLUSION

The minimal common region of chromosome deletion in periampullary neoplasms is defined between the D9S974 and D9S942 loci within a 15 kb interval in 9p21, suggesting the involvement of a novel tumor suppressor gene in their carcinogenesis.

摘要

目的

进一步明确壶腹周围肿瘤中9号染色体短臂2区1带（9p21）缺失的范围。

方法

采用聚合酶链反应（PCR）、聚丙烯酰胺凝胶电泳（PAGE）及银染法，检测35例壶腹周围肿瘤标本及其配对血样中9p21上5个微卫星多态性标记的杂合性缺失。

结果

50%（4/8）的胰腺癌病例在一个或多个微卫星位点出现杂合性缺失，其中D9S974位点缺失频率较高（37.5%），D9S942位点次之（28.6%），部分病例表现为连续等位基因缺失。62.5%（5/8）的壶腹癌病例在一个或多个位点出现杂合性缺失，缺失频率较高的位点为D9S942（42.9%），其次为IFNA（37.5%）和D9S171（37.5%）。14.2%（1/7）的胰岛素瘤病例出现一个位点缺失。