猿猴病毒40大T抗原在人脑肿瘤中与p53和视网膜母细胞瘤蛋白形成特异性复合物。

Simian virus 40 large tumor antigen forms specific complexes with p53 and pRb in human brain tumors.

作者信息

Zhen H, Zhang X, Zhang Z, Fei Z, He X, Liang J, Huang W, Liu X, Zhang P

机构信息

Department of Neurosurgery, Xijing Hospital, Institute of Neurosurgery of PLA, Fourth Military Medical University, Xi'an 710032, China.

出版信息

Chin Med J (Engl). 2001 Apr;114(4):382-6.

PMID:11780459

Abstract

OBJECTIVE

To study the role of simian virus 40 (SV40) early region gene coding product large tumor antigen (Tag) expression and the interaction between Tag and tumor suppressors p53 and pRb in human brain tumorigenesis.

METHODS

Tag was investigated by immunoprecipitation followed by silver staining and Western blot in 65 cases of human brain tumors and 8 cases of normal brain tissues. Tag-p53 and Tag-pRb complexes were screened in 18 and 15 Tag positive tumor tissues, respectively.

RESULTS

Tag was found in all 8 ependymomas and 2 choroid plexus papillomas, 90% of pituitary adenomas (9/10), 73% of astrocytomas (11/15), 70% of meningiomas (7/10), 50% of glioblastomas multiforme (4/8), 33% of medulloblastomas (2/6). 5 oligodendrogliomas, 1 pineocytoma, and 8 normal brain tissues were negative for Tag. Tag-p53 complex was detected in all 18 Tag positive tumors. Tag-pRb complex was found in all 15 Tag positive tumors.

CONCLUSION

SV40 Tag is expressed in human brain tumors and can form specific complexes with tumor suppressors p53 and pRb. The inactivation of p53 and pRb due to the formation of Tag-p53 and Tag-pRb complexes may be an important mechanism in the etiopathogenesis of human brain tumors.

摘要

目的

研究猿猴病毒40（SV40）早期区域基因编码产物大T抗原（Tag）的表达及其与肿瘤抑制因子p53和pRb的相互作用在人脑肿瘤发生中的作用。

方法

采用免疫沉淀结合银染和蛋白质印迹法检测65例人脑肿瘤和8例正常脑组织中的Tag。分别在18例和15例Tag阳性肿瘤组织中筛选Tag-p53和Tag-pRb复合物。

结果

在所有8例室管膜瘤、2例脉络丛乳头状瘤、90%的垂体腺瘤（9/10）、73%的星形细胞瘤（11/15）、70%的脑膜瘤（7/10）、50%的多形性胶质母细胞瘤（4/8）、33%的髓母细胞瘤（2/6）中检测到Tag。5例少突胶质细胞瘤、1例松果体细胞瘤和8例正常脑组织Tag呈阴性。在所有18例Tag阳性肿瘤中均检测到Tag-p53复合物。在所有15例Tag阳性肿瘤中均发现Tag-pRb复合物。