Ogasa A, Yokoki Y
Natl Inst Anim Health Q (Tokyo). 1975 Summer;15(2):94-102.
The role of testosterone in the early stage of spermatocytogenesis was investigated in newborn rats. The testes of rats, either 0 or 6 days of age, were implanted into those of hypophysectomized adult rats that had or had not been injected with testosterone propionate (TP) after hypophysectomy and also into those in intact adult rats. All the animals were autopsied 17 or 11 days later when the implanted testes reached 17 days of age. The implanted testes were examined for cellular components in the seminiferous tubules. In an additional experiment, newborn rats were injected with TP or cyproterone acetate, an antagonistic substance against androgen, daily for the first 17 days of life and examined for testes. Proliferation of supporting cells and development of seminiferous tubules were less remarkable in the testes of newborn rats which had been implanted into the testes of hypophysectomized rats than in those which had been implanted into the testes of intact adult rats. Proliferation of supporting cells was not stimulated by TP, but development of seminiferous tubules was slightly promoted. Progress in spermatocytogenesis from gonocytes to pachytene primary spermatocytes was observed in the testes of newborn rats which had been implanted into the testes of hypophysectiomized rats. It was not so marked after injection with TP. These results suggested that testosterone might have stimulated development of seminiferous tubules and maturation of spermatocytes in the early stage of spermatocytogenesis by its synergistic action with a gonadotropin, possible follicle-stimulating hormone.
在新生大鼠中研究了睾酮在精母细胞生成早期的作用。将0日龄或6日龄大鼠的睾丸植入垂体切除的成年大鼠的睾丸中，这些垂体切除的成年大鼠在垂体切除后已注射或未注射丙酸睾酮（TP），同时也将其植入完整成年大鼠的睾丸中。17天或11天后，当植入的睾丸达到17日龄时，对所有动物进行解剖。检查植入睾丸的生精小管中的细胞成分。在另一项实验中，新生大鼠在出生后的前17天每天注射TP或醋酸环丙孕酮（一种雄激素拮抗剂），然后检查睾丸。与植入完整成年大鼠睾丸的新生大鼠相比，植入垂体切除大鼠睾丸的新生大鼠睾丸中支持细胞的增殖和生精小管的发育不太明显。TP不能刺激支持细胞的增殖，但能轻微促进生精小管的发育。在植入垂体切除大鼠睾丸的新生大鼠睾丸中观察到了从生殖细胞到粗线期初级精母细胞的精母细胞生成进展。注射TP后这种进展不明显。这些结果表明，睾酮可能通过与促性腺激素（可能是促卵泡激素）的协同作用，刺激精母细胞生成早期生精小管的发育和精母细胞的成熟。
