Blackston C R, Dubey J P, Dotson E, Su C, Thulliez P, Sibley D, Lehmann T
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Chamblee, Georgia 30341, USA.
J Parasitol. 2001 Dec;87(6):1472-5. doi: 10.1645/0022-3395(2001)087[1472:HRTOTG]2.0.CO;2.
High-resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current typing methods discern 3 lineages with minimal within-lineage variation. Described here are 6 new variable loci. These loci, including a minisatellite and 5 microsatellites, were more polymorphic than allozymes, restriction fragment length polymorphisms, and sequence variation in introns. Most importantly, these loci revealed, for the first time, substantial within-lineage variation that was over 6-fold higher than that detected by other markers. Genotyping at these loci facilitates classification of isolates beyond the lineage level.
对弓形虫进行高分辨率分型对于理解不同菌株间的基因差异对疾病表现和传播模式变化的影响至关重要。目前的分型方法可识别出3个谱系，谱系内变异极小。本文描述了6个新的可变位点。这些位点，包括一个小卫星和5个微卫星，比同工酶、限制性片段长度多态性以及内含子中的序列变异具有更高的多态性。最重要的是，这些位点首次揭示了谱系内存在显著变异，其变异程度比其他标记检测到的高出6倍以上。在这些位点进行基因分型有助于在谱系水平之上对分离株进行分类。
