通过表达克隆鉴定人CD93为吞噬性C1q受体（C1qRp）。

Identification of human CD93 as the phagocytic C1q receptor (C1qRp) by expression cloning.

作者信息

Steinberger Peter, Szekeres Andreas, Wille Stefan, Stöckl Johannes, Selenko Nicole, Prager Elisabeth, Staffler Günther, Madic Otto, Stockinger Hannes, Knapp Walter

机构信息

Institute of Immunology, University of Vienna, A-1090 Vienna, Austria.

出版信息

J Leukoc Biol. 2002 Jan;71(1):133-40.

PMID:11781389

Abstract

CD93 is a approximately 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. So far, its primary structure and function were unknown. We used retroviral-expression cloning to isolate the CD93 cDNA. Sequence analysis revealed that CD93 is identical to a protein on human phagocytes termed C1q receptor (C1qRp). C1qRp was shown previously to mediate enhancement of phagocytosis in monocytes and was suggested to be a receptor of C1q and two other structurally related molecules. When studying CD93 transductants and control cells, we found that cells expressing CD93 have enhanced capacity to bind C1q. Furthermore, we show that immature dendritic cells (DC) express CD93/C1qRp, and mature DC, known to have reduced capacity for antigen uptake and to have lost the ability to phagocytose, show weak-to-negative CD93/C1qRp expression.

摘要

CD93是一种分子量约为120 kDa的O-唾液酸糖蛋白，在造血系统中，它在髓系谱系细胞上选择性表达。到目前为止，其一级结构和功能尚不清楚。我们利用逆转录病毒表达克隆技术分离出CD93 cDNA。序列分析表明，CD93与人吞噬细胞上的一种名为C1q受体（C1qRp）的蛋白质相同。先前已证明C1qRp可介导单核细胞吞噬作用的增强，并被认为是C1q和其他两种结构相关分子的受体。在研究CD93转导细胞和对照细胞时，我们发现表达CD93的细胞结合C1q的能力增强。此外，我们还表明，未成熟树突状细胞（DC）表达CD93/C1qRp，而成熟DC已知其抗原摄取能力降低且失去了吞噬能力，其CD93/C1qRp表达呈弱阳性至阴性。