Isoda K, Kamezawa Y, Tada N, Sato M, Ohsuzu F
First Department of Internal Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.
J Card Fail. 2001 Dec;7(4):355-64. doi: 10.1054/jcaf.2001.28221.
Interleukin (IL)-1 has profound effects on nonimmune cells and organs, including the heart. The effects of IL-1 on transgenic hearts have not yet been described.
We generated transgenic mice overexpressing the human IL-1 gene under control of the cytomegalovirus enhancer/chicken beta-actin promoter. Heart weight-body weight ratio increased 1.4- to 2.2-fold in transgenic mice compared with wild-type mice. Lung weight-body weight ratio also increased in transgenic mice, all of which died within 14 days of birth. Light microscopy revealed concentric hypertrophy with cardiomyocyte hypertrophy in all transgenic mice and pulmonary edema in some of them. Electron microscopy showed myofilament loss and an increased number of giant mitochondria, but no sarcomere disarray. Northern blotting showed that gene expression had been reprogrammed in the left ventricle of transgenic mice. Expression of fetal-type genes such as prepro-atrial natriuretic factor and beta-myosin heavy chain were increased, but voltage-dependent calcium channel messenger RNA expression was decreased in the left ventricle of transgenic mice compared with that of wild-type mice.
IL-1 may cause structural and functional alterations in cardiac myocytes.
白细胞介素（IL）-1 对包括心脏在内的非免疫细胞和器官有深远影响。IL-1 对转基因心脏的影响尚未见报道。
我们构建了在巨细胞病毒增强子/鸡β-肌动蛋白启动子控制下过表达人 IL-1 基因的转基因小鼠。与野生型小鼠相比，转基因小鼠的心脏重量与体重之比增加了 1.4 至 2.2 倍。转基因小鼠的肺重量与体重之比也增加，所有这些小鼠在出生后 14 天内死亡。光学显微镜显示所有转基因小鼠均有同心性肥大伴心肌细胞肥大，部分小鼠有肺水肿。电子显微镜显示肌丝丢失和巨大线粒体数量增加，但无肌节紊乱。Northern 印迹分析表明转基因小鼠左心室的基因表达已重新编程。与野生型小鼠相比，转基因小鼠左心室中诸如前心钠素原和β-肌球蛋白重链等胎儿型基因的表达增加，但电压依赖性钙通道信使核糖核酸表达降低。
IL-1 可能导致心肌细胞发生结构和功能改变。
