van Os C P, Rijke-Schilder G P, Vliegenthart J F
Biochim Biophys Acta. 1979 Dec 18;575(3):479-84. doi: 10.1016/0005-2760(79)90120-6.
Type-2 lipoxygenases from soybeans and peas, which have a pH optimum of 6--7 were examined for oxygenation activity at pH 9.0. The reaction velocity was found to be strongly dependent on substrate concentration. At higher substrate concentrations an inhibitory effect was observed, which is connected with the occurrence of a kinetic lag phase. On incubation of linoleic acid at pH 9.0 with either of these enzymes predominantly 9-LR-hydroperoxy-10-trans,12-cis-octadecadienoic acid is formed. The similarity of the product specificity with that of prostaglandin synthetase is discussed in view of the formation of prostaglandin-like substances by soybean lipoxygenase-2 (Bild, G.S., Bhat, S.G., Ramadoss, C.S. and Axelrod, B. (1978) J. Biol. Chem, 253, 21--23).
对来自大豆和豌豆的2型脂氧合酶进行了检测，其最适pH值为6 - 7，在pH 9.0条件下检测其加氧活性。发现反应速度强烈依赖于底物浓度。在较高底物浓度下观察到抑制作用，这与动力学延迟阶段的出现有关。在pH 9.0条件下，将亚油酸与这两种酶中的任何一种一起孵育，主要形成9-LR-氢过氧-10-反式，12-顺式-十八碳二烯酸。鉴于大豆脂氧合酶-2形成前列腺素样物质的情况，讨论了产物特异性与前列腺素合成酶的相似性（比尔德，G.S.，巴特，S.G.，拉马多斯，C.S.和阿克塞尔罗德，B.（1978年）《生物化学杂志》，253，21 - 23）。
