水稻胚芽脂氧合酶的产物特异性
Product specificity of rice germ lipoxygenase.
作者信息
Yamamoto A, Fujii Y, Yasumoto K, Mitsuda H
出版信息
Lipids. 1980 Jan;15(1):1-5. doi: 10.1007/BF02534108.
PMID:6767147
Abstract
Incubation of linoleic acid with partially purified lipoxygenase from rice germ yielded a ratio of 9- to 13-hydroperoxides of linoleic acid of 97:3 as measured by high performance liquid chromatography. Under similar conditions, hematin gave the 9- to 13-hydroperoxides at a ratio of 51:49, and soybean lipoxygenase-a at 9:91. Infrared spectral analysis revealed cis-trans configuration to predominate in the reaction products with the rice germ enzyme as was with the soybean enzyme.
摘要
用来自稻胚的部分纯化的脂氧合酶孵育亚油酸，通过高效液相色谱法测定，亚油酸9-氢过氧化物与13-氢过氧化物的比例为97:3。在类似条件下，血红素产生的9-氢过氧化物与13-氢过氧化物的比例为51:49，大豆脂氧合酶-a产生的比例为9:91。红外光谱分析表明，与大豆酶一样，稻胚酶反应产物中顺反构型占主导。
Zotero 插件