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用于龋齿风险评估的变异链球菌浸涂试验的效能

Efficacy of a dip slide test for mutans streptococci in caries risk assessment.

作者信息

Pinelli C, Serra M C, Loffredo L C

机构信息

Department of Restorative Dentistry, Faculty of Dentistry of Piracicaba, UNICAMP (University of Campinas), Avenida Limeira, 901, Areião, Piracicaba, São Paulo, Brazil.

出版信息

Community Dent Oral Epidemiol. 2001 Dec;29(6):443-8. doi: 10.1034/j.1600-0528.2001.290605.x.

DOI:10.1034/j.1600-0528.2001.290605.x
PMID:11784287
Abstract

The aim of this study was to evaluate the efficacy of a dip slide test for mutans streptococci in caries risk assessment, when the microbiological results were compared to well-defined clinical criteria (DCC) for caries risk, clinically measured through high and low caries activity. Eighty-one volunteers from the 6th to 8th grades from public schools of Piracicaba, São Paulo, Brazil, were evaluated for dental caries. All free smooth surfaces were evaluated to check whether or not there were white spots. Based on the subjects' caries experience, a calibrated clinician divided them into groups of high and low caries activity. The subjects were submitted to a salivary test (CARITEST SM) from the same batch number. Kappa statistics (kappa) were applied to verify the reproducibility of the simplified test, checked through interexaminer agreement when the results were classified by independent and blind means. The microbiological results were validated according to expressions of sensitivity and specificity. A moderate agreement was verified as the results were classified according to 6 scores (kappa=0.55), and the agreement was substantial when the results were classified according to high and low microbiological count (kappa=0.78). The sensitivity and specificity values were 0.59 and 0.85, respectively, showing that the test was more specific than sensitive, and could thus better identify the low caries risk subjects.

摘要

本研究的目的是评估在将微生物学结果与通过高、低龋活性临床测量的明确龋风险临床标准(DCC)进行比较时,用于变形链球菌的浸片试验在龋风险评估中的功效。对来自巴西圣保罗皮拉西卡巴公立学校6至8年级的81名志愿者进行了龋齿评估。对所有游离光滑面进行评估,检查是否存在白斑。根据受试者的龋经验,一名经过校准的临床医生将他们分为高龋活性组和低龋活性组。受试者接受了来自同一批号的唾液检测(CARITEST SM)。应用kappa统计量(kappa)来验证简化试验的可重复性,当结果通过独立和盲法分类时,通过检查者间一致性进行检查。根据敏感性和特异性的表达对微生物学结果进行验证。当结果根据6个评分进行分类时,验证了中度一致性(kappa = 0.55),当结果根据微生物计数的高和低进行分类时,一致性很强(kappa = 0.78)。敏感性和特异性值分别为0.59和0.85,表明该试验特异性高于敏感性,因此能够更好地识别低龋风险受试者。

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