Lee K, Flegal C J, Wolford J H
Poult Sci. 1975 Mar;54(2):374-80. doi: 10.3382/ps.0540374.
A series of four experiments was conducted with a commercial strain of laying chickens in an attempt to experimentally produce Fatty Liver-Hemorrhagic syndrome (FLHS). Different dietary energy sources, environmental temperatures and feeding schedules were the experimental variables. Increasing the environmental temperature from 12.2 degrees C. (experiment 1) to 27.8 degrees C. (experiment 2) did not drastically alter total liver fat, 2.7 g. (experiment 1) vs. 2.9 g. (experiment 2) or the incidence of FLHS, 0.0% (experiment 1) vs. 1.0% (experiment 2). Likewise, in experiment 4, changing the environmental temperature from 22.2 degrees C. to 30.6 degrees C. or from 30.6 degrees C. to 22.2 degrees C. did not significantly (P greater than .05) alter total liver fat or liver wet weight. The greatest incidence (50%) of FLHS occurred in birds which were restricted in feed intake and exposed to an environmental temperature of 30.6 degrees C. from 32-36 weeks of age followed by ad libitum feeding at a temperature of 22.2 degrees C. from 36-40 weeks of age. These birds ate 26.8% more feed than the control birds during the ad libitum feeding period. Restricted feed schedules (experiment 3) resulted in significantly (P less than .05) lower 40 week liver wet weights and total fat when applied during the growing (14-20 weeks of age) and early lay (20-28 weeks of age) periods.
对一个商业蛋鸡品种进行了一系列四项实验，试图通过实验诱导出脂肪肝出血综合征（FLHS）。不同的膳食能量来源、环境温度和饲喂方案为实验变量。将环境温度从12.2摄氏度（实验1）提高到27.8摄氏度（实验2），并未显著改变肝脏总脂肪量，实验1为2.7克，实验2为2.9克，也未改变FLHS的发病率，实验1为0.0%，实验2为1.0%。同样，在实验4中，将环境温度从22.2摄氏度变为30.6摄氏度或从30.6摄氏度变为22.2摄氏度，也未显著（P大于0.05）改变肝脏总脂肪量或肝脏湿重。FLHS的最高发病率（50%）出现在那些在32至36周龄时采食量受限且暴露于30.6摄氏度环境温度下，随后在36至40周龄时在22.2摄氏度温度下自由采食的鸡群中。在自由采食期，这些鸡比对照鸡多吃26.8%的饲料。在生长阶段（14至20周龄）和产蛋初期（20至28周龄）采用限制饲喂方案（实验3），会导致40周龄时肝脏湿重和总脂肪量显著（P小于0.05）降低。
