内皮素-3对肠道离子转运的影响。

Effects of endothelin-3 on intestinal ion transport.

作者信息

González Bosc L V, Majowicz M P, Ortiz M C, Vidal N A

机构信息

Cátedra de Biología Celular e Histología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Junín 956, 1er piso (1113), Buenos Aires, Argentina.

出版信息

Peptides. 2001 Dec;22(12):2069-75. doi: 10.1016/s0196-9781(01)00554-x.

DOI:10.1016/s0196-9781(01)00554-x

PMID:11786192

Abstract

We investigated the effects of endothelin 3 (ET-3) on electrolyte transport in rat small intestine using a voltage clamp technique in Ussing's chamber. ET-3 diminished potential difference (PD) and short circuit current (Isc). ET-3 did not affect PD or Isc in low Na(+) and/or D-glucose-free medium. Phloridzine (an inhibitor of sodium-glucose cotransporter [SGLT1]) pretreatment abolished the effect of ET-3 on Isc. Methylene blue (a soluble guanylate cyclase inhibitor) or N-nitro-L-arginine methyl ester (a NOS inhibitor) pretreatment delayed the effect of ET-3 on PD and Isc. ET-3 enhanced NOS activity on enterocytes and systemic NO production. Then, ET-3 could inhibit SGLT1 with the participation of NO.

摘要

我们使用尤斯灌流小室中的电压钳技术研究了内皮素3（ET-3）对大鼠小肠电解质转运的影响。ET-3降低了电位差（PD）和短路电流（Isc）。在低钠和/或无D-葡萄糖的培养基中，ET-3不影响PD或Isc。根皮苷（钠-葡萄糖共转运体[SGLT1]抑制剂）预处理消除了ET-3对Isc的影响。亚甲蓝（一种可溶性鸟苷酸环化酶抑制剂）或N-硝基-L-精氨酸甲酯（一种一氧化氮合酶抑制剂）预处理延迟了ET-3对PD和Isc的影响。ET-3增强了肠细胞上的一氧化氮合酶活性和全身一氧化氮的产生。因此，ET-3可在一氧化氮的参与下抑制SGLT1。