Zhou Xin, Potoka Douglas A, Boyle Patricia, Nadler Evan P, McGinnis Kathleen, Ford Henri R
Department of Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, PA, USA.
FEMS Microbiol Lett. 2002 Jan 2;206(1):93-7. doi: 10.1111/j.1574-6968.2002.tb10992.x.
Aminoguanidine (AG), a nitric oxide synthase (NOS) inhibitor, has been widely used to study the role of inducible NOS (iNOS) in host defense against infections caused by various pathogens including Salmonella typhimurium. iNOS has been reported to play an important role in host defense against S. typhimurium infection both in vitro and in vivo. In this report we show those AG treatment lead to weight loss in both wild-type and iNOS knockout mice, and rendered them more susceptible to Salmonella infection. These results suggest that AG may have side effects other than the inhibition of iNOS, and that data obtained from studies using AG should be interpreted with caution.
氨基胍（AG）是一种一氧化氮合酶（NOS）抑制剂，已被广泛用于研究诱导型一氧化氮合酶（iNOS）在宿主抵御包括鼠伤寒沙门氏菌在内的各种病原体感染中的作用。据报道，iNOS在体外和体内对鼠伤寒沙门氏菌感染的宿主防御中均发挥重要作用。在本报告中，我们表明，AG处理会导致野生型和iNOS基因敲除小鼠体重减轻，并使它们更容易受到沙门氏菌感染。这些结果表明，AG可能除了抑制iNOS之外还有其他副作用，并且从使用AG的研究中获得的数据应谨慎解读。
