Ohta S, Tabuchi Y, Yanai N, Asano S, Fuse H, Obinata M
Department of Urology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama City, Japan.
Arch Androl. 2002 Jan-Feb;48(1):43-51. doi: 10.1080/014850102753385206.
A Leydig cell line, TTE1, has been established from transgenic mice harboring a temperature-sensitive simian virus 40 (tsSV40) large T-antigen gene. The cells grew at a permissive temperature (33 degrees C), but growth was markedly prevented at a nonpermissive temperature (39 degrees C). T-antigen was expressed in the nuclei at 33 degrees C but disappeared at 39 degrees C, indicating that the cells show a temperature-sensitive growth phenotype reflected by the tsSV40 large T-antigen. TTE1 cells did not show any colony-forming activity in soft agar and form tumors in subcutaneous tissue in nude mice, indicating that the cells were not transformed. Alkaline phosphatase and 3beta-hydroxysteroid dehydrogenase (HSD) activities or expression of cytokeratin and vimentin were observed. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that TTE1 cells expressed mRNAs encoding 17beta-HSD types 1 and 3, and inhibin-alpha. The cells with unique characteristics, therefore, should serve useful model study the function of Leydig cell.
一种莱迪希细胞系TTE1是从携带温度敏感型猿猴病毒40（tsSV40）大T抗原基因的转基因小鼠中建立的。这些细胞在允许温度（33℃）下生长，但在非允许温度（39℃）下生长明显受到抑制。T抗原在33℃时在细胞核中表达，但在39℃时消失，这表明这些细胞呈现出由tsSV40大T抗原反映的温度敏感型生长表型。TTE1细胞在软琼脂中未表现出任何集落形成活性，且在裸鼠皮下组织中不形成肿瘤，表明这些细胞未发生转化。观察到了碱性磷酸酶和3β-羟基类固醇脱氢酶（HSD）活性或细胞角蛋白和波形蛋白的表达。逆转录-聚合酶链反应（RT-PCR）分析表明，TTE1细胞表达编码17β-HSD 1型和3型以及抑制素α的mRNA。因此，具有独特特征的这些细胞应该会成为研究莱迪希细胞功能的有用模型。
