黑腹果蝇87A7和87C1热诱导基因座的遗传与分子分析

Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster.

作者信息

Ish-Horowicz D, Pinchin S M, Schedl P, Artavanis-Tsakonas S, Mirault M E

出版信息

Cell. 1979 Dec;18(4):1351-8. doi: 10.1016/0092-8674(79)90245-9.

Abstract

Two different types of heat-inducible sequences are found at the cytogenetic loci 87A7 and 87C1 of D. melanogaster. One of these codes for the 70,000 dalton heat shock protein (hsp 70) and is found at both loci. The other type of sequence (alpha beta) codes for an RNA of unknown function and is found only at 87C1. We have completed a study of the organization of the two loci, using deficiencies that delete one or other locus, and have estimated the number of the hsp 70 genes at each locus. Thus in at least three strains of files there are a total of five coding sequences, three at 87C1 and two at 87A7. Restriction mapping of the coding regions at the two loci reveals that each of the two cytogenetic loci has its own characteristic coding sequence. The overall organization of the two loci appears to differ considerably. The alpha beta and hsp 70 heat-induced sequences at 87C1 are closely linked and are contained within two Eco RI restriction fragments.

摘要

在黑腹果蝇的细胞遗传学位点87A7和87C1发现了两种不同类型的热诱导序列。其中一种编码70,000道尔顿的热休克蛋白（hsp 70），在两个位点均有发现。另一种序列类型（αβ）编码一种功能未知的RNA，仅在87C1位点发现。我们利用缺失一个或另一个位点的缺失系，完成了对这两个位点组织结构的研究，并估计了每个位点hsp 70基因的数量。因此，在至少三个果蝇品系中共有五个编码序列，三个在87C1，两个在87A7。对这两个位点编码区的限制性图谱分析表明，两个细胞遗传学位点各自都有其独特的编码序列。这两个位点的整体组织结构似乎有很大差异。87C1位点的αβ和hsp 70热诱导序列紧密相连，包含在两个Eco RI限制性片段内。