Kurosu Katsushi, Yumoto Norio, Rom William N, Takiguchi Yuichi, Jaishree Jagirdai, Nakata Koh, Tatsumi Koichiro, Mikata Aatsuo, Kuriyama Takatyuki, Weiden Michael D
Department of Respirology, School of Medicine, Chiba University, Chiba, Japan.
Am J Respir Crit Care Med. 2002 Jan 15;165(2):254-9. doi: 10.1164/ajrccm.165.2.2101141.
We used a denaturing gradient gel electrophoresis (DGGE) procedure with 40-nucleotide guanine- and cytosine-rich sequences in the polymerase chain reaction (PCR) and sequencing analysis to analyze the T cell antigen receptor (TCR)-Vgamma gene repertoire of infiltrating T lymphocytes in pulmonary lymphoproliferative disorders. Six of 15 low-grade mucosa-associated lymphoid tissue (MALT) lymphomas and 8 of 15 cases of lymphocytic interstitial pneumonia (LIP) showed some oligoclonal bands for TCR-Vgamma genes on DGGE. Sequencing analysis demonstrated plural oligoclonal TCR-Vgamma clones among the oligoclonal PCR products on DGGE, leading to the conclusion that conventional antigen-specific oligoclonal expansions may play some role in the pathogenesis of pulmonary lymphoproliferative disorders. The frequency of oligoclonal infiltrating T cell expansions in human immunodeficiency virus (HIV)-related LIP (100%) was significantly higher than in low-grade pulmonary MALT lymphomas (40%) or in HIV-negative LIP (30%). Because recent evidence demonstrates that the V3 loop in the proviral amino acid sequences of mononuclear cells from bronchoalveolar lavage is more homogeneous than those from peripheral blood, this homogeneity might result in oligoclonal expansions of infiltrating T lymphocytes as a consequence of ongoing reactions against lung-specific viral strains.
我们采用变性梯度凝胶电泳（DGGE）方法，在聚合酶链反应（PCR）中使用富含鸟嘌呤和胞嘧啶的40个核苷酸序列，并进行测序分析，以分析肺淋巴增殖性疾病中浸润性T淋巴细胞的T细胞抗原受体（TCR）-Vγ基因谱。15例低度黏膜相关淋巴组织（MALT）淋巴瘤中的6例以及15例淋巴细胞间质性肺炎（LIP）中的8例，在DGGE上显示出TCR-Vγ基因的一些寡克隆条带。测序分析表明，DGGE上的寡克隆PCR产物中有多个寡克隆TCR-Vγ克隆，由此得出结论，传统的抗原特异性寡克隆扩增可能在肺淋巴增殖性疾病的发病机制中起一定作用。人类免疫缺陷病毒（HIV）相关LIP中寡克隆浸润性T细胞扩增的频率（100%）显著高于低度肺MALT淋巴瘤（40%）或HIV阴性LIP（30%）。因为最近的证据表明，支气管肺泡灌洗单核细胞原病毒氨基酸序列中的V3环比外周血单核细胞的更具同源性，这种同源性可能是由于针对肺特异性病毒株的持续反应导致浸润性T淋巴细胞寡克隆扩增的结果。
