Sandhu Kulmeet K, McIntosh Catherine M, Simard Joseph M, Smith Sallie W, Rotello Vincent M
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Bioconjug Chem. 2002 Jan-Feb;13(1):3-6. doi: 10.1021/bc015545c.
Mixed monolayer protected gold clusters (MMPCs) functionalized with quaternary ammonium chains efficiently transfect mammalian cell cultures, as determined through beta-galactosidase transfer and activity. The success of these transfection assemblies depended on several variables, including the ratio of DNA to nanoparticle during the incubation period, the number of charged substituents in the monolayer core, and the hydrophobic packing surrounding these amines. Complexes of MMPCs and plasmid DNA formed at w/w ratios of 30 were most effective in promoting transfection of 293T cells in the presence of 10% serum and 100 microM chloroquine. The most efficient nanoparticle studied (MMPC 7) was approximately 8-fold more effective than 60 kDa polyethylenimine, a widely used transfection agent.
通过β-半乳糖苷酶转移和活性测定，用季铵链功能化的混合单层保护金簇（MMPC）能有效地转染哺乳动物细胞培养物。这些转染组装体的成功取决于几个变量，包括孵育期内DNA与纳米颗粒的比例、单层核心中带电取代基的数量以及这些胺周围的疏水堆积。在10%血清和100微摩尔氯喹存在的情况下，以30的w/w比例形成的MMPC与质粒DNA复合物在促进293T细胞转染方面最有效。所研究的最有效的纳米颗粒（MMPC 7）比广泛使用的转染剂60 kDa聚乙烯亚胺的效果大约高8倍。
