Konomi Hiroyuki, Meedeniya Adrian C B, Simula Maria E, Toouli James, Saccone Gino T P
Department of General and Digestive Surgery and the Centre for Neuroscience, Flinders University of South Australia, Adelaide, South Australia 5001, Australia.
J Comp Neurol. 2002 Jan 28;443(1):15-26. doi: 10.1002/cne.10094.
The motor innervation of the duodenum and distal colon remains uncharacterized within the same species. Our aim was to compare the projections and neurochemical properties of duodenal and distal colon circular muscle motor neurons. Circular muscle motor neurons were retrogradely traced by using a neural tracer in vitro, processed for choline acetyltransferase (ChAT) and nitric oxide synthase (NOS) immunoreactivity and then visualized by using indirect immunofluorescence. A mean of 372 +/- 64 and 156 +/- 23 neurons (mean +/- SEM) were tracer-labeled within the duodenum and colon, respectively. The ChAT+/NOS- neurons comprised 57.6 +/- 6.6% and 39.6 +/- 4.4% of all labeled cells in the duodenum and colon, respectively, and projected mainly in the oral direction. Of all labeled cells, the ChAT-/NOS+ neurons comprised 8.5 +/- 2.3% in the duodenum and 46.6 +/- 5.0% in the distal colon and projected mainly in the anal direction. Of the remainder, 20.6 +/- 5.0% and 8.2 +/- 2.4% were ChAT+/NOS+ and 13.2 +/- 0.9% and 5.6 +/- 1.4% were ChAT-/NOS- in the duodenum and distal colon, respectively. Within both regions, the distribution of the ChAT+/NOS- and ChAT-/NOS+ neurons are consistent with the ascending excitatory and descending inhibitory reflexes. The proportion of ChAT-/NOS+ neurons is greater within the colon in comparison with the duodenum. A considerable proportion of duodenal motor neurons were ChAT+/NOS+ and ChAT-/NOS-. These two classes may underlie nonperistaltic motor patterns, which predominate within the duodenum. These findings demonstrate regional differences in the innervation of intestinal circular muscle.
在同一物种中，十二指肠和远端结肠的运动神经支配情况仍未明确。我们的目的是比较十二指肠和远端结肠环行肌运动神经元的投射和神经化学特性。通过在体外使用神经示踪剂对环行肌运动神经元进行逆行追踪，然后进行胆碱乙酰转移酶（ChAT）和一氧化氮合酶（NOS）免疫反应处理，最后使用间接免疫荧光进行可视化。在十二指肠和结肠中，分别平均有372±64和156±23个神经元被示踪剂标记。ChAT+/NOS-神经元分别占十二指肠和结肠中所有标记细胞的57.6±6.6%和39.6±4.4%，主要向口腔方向投射。在所有标记细胞中，ChAT-/NOS+神经元在十二指肠中占8.5±2.3%，在远端结肠中占46.6±5.0%，主要向肛门方向投射。其余的ChAT+/NOS+神经元在十二指肠和远端结肠中分别占20.6±5.0%和8.2±2.4%，ChAT-/NOS-神经元在十二指肠和远端结肠中分别占13.2±0.9%和5.6±1.4%。在这两个区域内，ChAT+/NOS-和ChAT-/NOS+神经元的分布与上升的兴奋性和下降的抑制性反射一致。与十二指肠相比，结肠中ChAT-/NOS+神经元的比例更高。相当一部分十二指肠运动神经元是ChAT+/NOS+和ChAT-/NOS-。这两类神经元可能构成十二指肠中占主导的非蠕动运动模式的基础。这些发现表明肠道环行肌神经支配存在区域差异。
