Cano M, Arnold L L, Cohen S M
Department of Pathology and Microbiology and the Eppley Institute for Research on Cancer, University of Nebraska Medical Center, Omaha 68198-3135, USA.
Toxicol Pathol. 2001 Nov-Dec;29(6):600-6. doi: 10.1080/019262301753385924.
Few studies have examined the carcinogenicity of chemicals toward the urinary bladder in hamsters, and the effect of diet on hamster urine and urothelium has not been reported. Our laboratory recently began investigating the effects of dimethylarsinic acid (DMA) on the hamster bladder, and we noticed subtle urothelial changes even in controls. The possible effect of various diets on hamster urothelium was evaluated by feeding different diets to 4-week-old Syrian Golden hamsters for 5 weeks. The diets examined were Tekland 8656, Purina 5002, Purina 5L79, and NIH-07. Light microscopic examination showed a slight increase in urothelial hyperplasia in hamsters fed Purina 5L79. An increase in the incidence of urinary bladder necrosis, exfoliation, and mild hyperplasia were noted by scanning electron microscopy (SEM) with all dietary preparations except NIH-07. The constituents in the diets producing the urothelial alterations are not known at present, but NIH-07 diet was chosen for experiments to investigate the effects of DMA on the hamster bladder epithelium. Male and female 5-week-old Syrian Golden hamsters were fed 100 ppm DMA for 10 weeks. Examination of urinary parameters showed no treatment-related changes. Light microscopic examination and SEM revealed no changes of the urothelium of DMA-treated male or female hamsters.
很少有研究检测化学物质对仓鼠膀胱的致癌性，并且饮食对仓鼠尿液和膀胱上皮的影响尚无报道。我们实验室最近开始研究二甲基胂酸（DMA）对仓鼠膀胱的影响，我们注意到即使在对照组中也有细微的膀胱上皮变化。通过给4周龄的叙利亚金黄仓鼠喂食不同的饮食5周，评估了各种饮食对仓鼠膀胱上皮的可能影响。所检测的饮食包括Tekland 8656、普瑞纳5002、普瑞纳5L79和NIH - 07。光学显微镜检查显示，喂食普瑞纳5L79的仓鼠膀胱上皮增生略有增加。除NIH - 07外，所有饮食组的扫描电子显微镜（SEM）检查均发现膀胱坏死、脱落和轻度增生的发生率增加。目前尚不清楚导致膀胱上皮改变的饮食成分，但选择NIH - 07饮食用于研究DMA对仓鼠膀胱上皮影响的实验。给5周龄的雄性和雌性叙利亚金黄仓鼠喂食100 ppm的DMA，持续10周。尿液参数检查未发现与处理相关的变化。光学显微镜检查和SEM均未发现经DMA处理的雄性或雌性仓鼠膀胱上皮有变化。
