反义展示：一种用于功能基因筛选的新方法及其在血管生成相关基因分离中的应用。

Antisense display: a new method for functional gene screen and its application to angiogenesis-related gene isolation.

作者信息

Yonekura H, Yasui K, Sakurai S, Li H, Yamamoto Y, Yamamoto H

机构信息

Department of Biochemistry and Molecular Vascular Biology, Kanazawa University Graduate School of Medical Science, Japan.

出版信息

Ann N Y Acad Sci. 2001 Dec;947:382-6. doi: 10.1111/j.1749-6632.2001.tb03968.x.

DOI:10.1111/j.1749-6632.2001.tb03968.x

PMID:11795296

Abstract

We have developed an antisense-oriented method for functional gene screening, which we termed "antisense display." After evaluation in a cell-free system, we applied this method to a cellular system for isolating angiogenesis-related genes and came across a gene previously not described in humans. A screen of 10 mer antisense repertoire led to the identification of a unique 'angiostatic' antisense, and the gene corresponding to it was found to be preferentially expressed in vascular endothelial cells, coding for a putative type-2 membrane protein containing Rab-GAP/TBC and EF-hand-like domains. We named it vascular Rab-GAP/TBC domain-containing protein (VRP). The results obtained thus indicate that the antisense display method is useful for the isolation of functional genes responsible for phenotypes concerned.

摘要

我们开发了一种用于功能基因筛选的反义导向方法，我们将其称为“反义展示”。在无细胞系统中进行评估后，我们将该方法应用于细胞系统以分离血管生成相关基因，并发现了一个以前在人类中未被描述的基因。对10聚体反义文库的筛选导致鉴定出一种独特的“血管抑制”反义，并且发现与之对应的基因在血管内皮细胞中优先表达，编码一种含有Rab-GAP/TBC和EF-手样结构域的推定的2型膜蛋白。我们将其命名为含血管Rab-GAP/TBC结构域蛋白（VRP）。因此获得的结果表明，反义展示方法可用于分离负责相关表型的功能基因。