Yao Y C, Xiong J, Wang X M, Li J X, Hu Y P
Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.
Yi Chuan Xue Bao. 2001;28(12):1116-9.
To explore the potentiality of the mouse ES cell line which is employed in our laboratory were transfected with neo gene and selected with G418. Some ES cell clones expressing neo gene were obtained. After ES clones were picked up and expanded, the ES cells were mircoinjected into blastocysts and implanted miceuterus. 60 blastocysts were implanted into 5 mice. Finally, 3 chimeric mice with neo gene were born. PCR was carried out to analyze the presentation of neo gene in tissues of chimeric mice. The results showed that many tissues harbored the neo gene, including skin, liver and blood et al.
为了探索我们实验室所使用的小鼠胚胎干细胞系的潜能，用新霉素基因转染这些细胞并用G418进行筛选。获得了一些表达新霉素基因的胚胎干细胞克隆。挑选并扩增胚胎干细胞克隆后，将胚胎干细胞显微注射到囊胚中并植入小鼠子宫。将60个囊胚植入5只小鼠体内。最终，3只携带新霉素基因的嵌合小鼠出生。进行聚合酶链反应（PCR）以分析嵌合小鼠组织中新霉素基因的表达情况。结果显示，许多组织都含有新霉素基因，包括皮肤、肝脏和血液等。
