Obradovic M M, Stojimirovic B B, Trpinac D P, Milutinovic D D, Obradovic D I, Nesic V B
Institute of Histology and Embryology, University School of Medicine, Belgrade.
Srp Arh Celok Lek. 2001 Jul-Aug;129(7-8):175-9.
The introduction of peritoneal dialysis (PD) as a respectable modality of renal replacement therapy some three decades ago, suddenly drew attention of many authors to peritoneal membrane as insufficiently investigated structure. In order to explain the pathological changes in peritoneum due to renal diseases, it became necessary to explore the normal peritoneal structure. The aim of this study was to examine the morphology of peritoneal lining cells in healthy persons. Biopsies of the peritoneum were performed on 20 volunteer kidney donors. Tissue samples were taken during renal transplantation. Special care was taken in getting appropriate samples without artificial damage because of the extreme fragility of the peritoneal tissue. The preparing procedure was standard for routine HE staining and for plastic embedded semifine and fine sections studies. Semifine sections were made on ultramicrotome, stained with Toluidin blue and studied by light microscope, while fine sections were made by ultramicrotome and studied by transmission electron microscope. One layer of cuboidal or flattened lining cells present over the lamina propria connective tissue presented mesothelium. The cells were overlapped like tiles on the roof. Lateral parts of their interdigitated membranes were interconnected with different types of cell junctions: unpermeable, adhesion and communication junctions; inhibiting intercellular transport. Cell surface was often covered with great number of microvilli and lamellar bodies. A single kinocilia was also often present on apical cell surface. Nuclei were euchromatic with well developed nucleoli. Cytoplasm was filled with a great number of ribosomes, mitochondria, cisterns of rough endoplasmatic reticulum and Golgi apparatus, lamellar bodies and lipid inclusions. Numerous pinocytic vesicles on all parts of the membrane as well as in the cytoplasm indicating active endocytosis, egsocytosis and transcytosys in the process of secretion and reabsorption of serous liquid in peritoneal cavity, were visible. Euchromatic nuclei with prominent nucleoli and numerous mitochondria indicate cells of great metabolic activity.
大约三十年前，腹膜透析（PD）作为一种受认可的肾脏替代治疗方式被引入，这突然引起了许多作者对腹膜这一研究尚不充分的结构的关注。为了解释肾脏疾病导致的腹膜病理变化，有必要探究正常的腹膜结构。本研究的目的是检查健康人的腹膜衬里细胞形态。对20名自愿肾脏捐献者进行了腹膜活检。组织样本在肾脏移植期间采集。由于腹膜组织极其脆弱，在获取合适样本时格外小心，避免人为损伤。制备程序对于常规苏木精-伊红（HE）染色以及塑料包埋半薄和超薄切片研究而言是标准的。半薄切片在超薄切片机上制作，用甲苯胺蓝染色并通过光学显微镜观察，而超薄切片由超薄切片机制作并通过透射电子显微镜观察。在固有层结缔组织上存在一层立方形或扁平的衬里细胞，即间皮。细胞像屋顶上的瓦片一样相互重叠。它们相互交错的膜的侧面部分通过不同类型的细胞连接相互连接：不可渗透连接、黏附连接和通讯连接；抑制细胞间运输。细胞表面常常覆盖着大量微绒毛和板层小体。单个动纤毛也常常出现在细胞顶端表面。细胞核为常染色质，核仁发育良好。细胞质中充满了大量核糖体、线粒体、粗面内质网池和高尔基体、板层小体以及脂质包涵体。在膜的所有部分以及细胞质中可见大量胞饮小泡，这表明在腹膜腔浆液分泌和重吸收过程中存在活跃的胞吞作用、胞吐作用和跨细胞转运。常染色质细胞核伴有突出的核仁以及大量线粒体表明细胞具有很高的代谢活性。
