一氧化氮供体和一氧化氮合酶抑制剂对清醒绵羊瘤胃收缩的影响。
Effects of nitric oxide donor and nitric oxide synthase inhibitor on ruminal contractions in conscious sheep.
作者信息
Onaga T, Okada H, Hagiwara S, Nagashima C, Inoue H, Korczynski W, Kato S
机构信息
Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
出版信息
Res Vet Sci. 2001 Dec;71(3):189-95. doi: 10.1053/rvsc.2001.0507.
The present study was planned to evaluate a role of nitric oxide (NO) in the regulation of regular ruminal contractions in conscious sheep. Intravenous infusion of S-nitroso-acetyl-DL-penicillamine (SNAP) at doses of 3-30 nmol kg(-1) min(-1)for 30 minutes inhibited both the amplitude and frequency of ruminal contractions in a dose-dependent manner. However, intravenous infusion of Nomega-nitro-L-arginine-methyl ester (L-NAME) at doses of 0.3-3.0 micromol kg(-1) min(-1)did not alter the basal tone of intraruminal pressure and the amplitude of ruminal contractions. The frequency of contractions was slightly inhibited by L-NAME infusion at 1.0 micromol kg(-1)min(-1). The effects of L-NAME were abolished by simultaneous infusion of L -arginine at 30 micromol kg(-1) min(-1). These results suggest that exogenous NO can diminish the ruminal contractions, while endogenous NO is not involved in the regulatory mechanism of basal tone and regular phasic contractions of the rumen in healthy sheep.
本研究旨在评估一氧化氮（NO）在调节清醒绵羊瘤胃正常收缩中的作用。以3 - 30 nmol kg⁻¹ min⁻¹的剂量静脉输注S-亚硝基乙酰-DL-青霉胺（SNAP）30分钟，可剂量依赖性地抑制瘤胃收缩的幅度和频率。然而，以0.3 - 3.0 μmol kg⁻¹ min⁻¹的剂量静脉输注Nω-硝基-L-精氨酸甲酯（L-NAME），并未改变瘤内压的基础张力和瘤胃收缩的幅度。在1.0 μmol kg⁻¹ min⁻¹剂量下输注L-NAME，收缩频率略有抑制。同时以30 μmol kg⁻¹ min⁻¹的剂量输注L-精氨酸可消除L-NAME的作用。这些结果表明，外源性NO可减弱瘤胃收缩，而内源性NO不参与健康绵羊瘤胃基础张力和正常阶段性收缩的调节机制。
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