用于快速检测产气荚膜梭菌和败血梭菌的16S-23S rDNA间隔区扩增

Amplification of the 16S-23S rDNA spacer region for rapid detection of Clostridium chauvoei and Clostridium septicum.

作者信息

Sasaki Y, Yamamoto K, Amimoto K, Kojima A, Ogikubo Y, Norimatsu M, Ogata H, Tamura Y

机构信息

National Veterinary Assay Laboratory, 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan.

出版信息

Res Vet Sci. 2001 Dec;71(3):227-9. doi: 10.1053/rvsc.2001.0495.

DOI:10.1053/rvsc.2001.0495

PMID:11798300

Abstract

Amplification of the 16S-23S rDNA spacer region by polymerase chain reaction (PCR) was used for the rapid detection of Clostridium chauvoei and C septicum. To assess its specificity, PCR was performed with total DNA from 42 strains of clostridia and three strains of other genera. PCR products specific to C chauvoei or to C septicum were generated from homologous cultures only. Clostridium chauvoer-specific or C septicum-specific amplicons were also generated from tissues of cows experimentally infected with C chauvoei or C septicum and in DNA samples from cows clinically diagnosed as having blackleg or malignant oedema. These results suggest that a species-specific PCR may be useful for the rapid and direct detection of C chauvoei and C septicum in clinical specimens.

摘要

采用聚合酶链反应（PCR）扩增16S - 23S rDNA间隔区，用于快速检测产气荚膜梭菌和败血梭菌。为评估其特异性，用42株梭菌和3株其他属细菌的总DNA进行PCR。仅从同源培养物中产生了产气荚膜梭菌或败血梭菌特异的PCR产物。从实验感染产气荚膜梭菌或败血梭菌的奶牛组织以及临床诊断为黑腿病或恶性水肿的奶牛DNA样本中，也产生了产气荚膜梭菌特异或败血梭菌特异的扩增子。这些结果表明，种特异性PCR可能有助于临床标本中快速直接检测产气荚膜梭菌和败血梭菌。