Feng Z, Zhou Y, Jia Z, Lian J, Li J, Li W
Department of Infectious Diseases, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038.
Zhonghua Nei Ke Za Zhi. 1999 Jul;38(7):462-5.
To study the feasibility of DNA immunization with recombinant expression plasmid containing hepatitis C virus (HCV) core gene against HCV infection.
The recombinant plasmid containing HCV C gene was constructed and expressed transiently by using lipofectamine in the mouse SP2/0 cells. After appraisal and purification, these plasmid and control DNA were directly injected intramuscularly into BALB/c mice, HCV C specific antibody in the sera was checked with ELISA. Proliferative responses of spleen cells from experimental and control mice were determined by (3)H thymidine incorporation and cytotoxicity T lymphocyte (CTL) activity was measured by standard 4-h (51)Cr release assays.
All immunized mice developed anti-HCV core antibodies and this antibody was dose and immune number dependent. Lymphoproliferative responses for HCV core antigen were higher in immunized mice than in control mice (P < 0.05), stimulation indices (SI) were 2.42 - 6.10. CTLs might induce approximately 63.13% lysis of pcDNA HCV C transfected SP2/0 target cells.
It is suggested that HCV C gene immunization is a promising method of vaccination against HCV infection.
研究含丙型肝炎病毒（HCV）核心基因的重组表达质粒进行DNA免疫以预防HCV感染的可行性。
构建含HCV C基因的重组质粒，利用脂质体在小鼠SP2/0细胞中进行瞬时表达。经鉴定和纯化后，将这些质粒和对照DNA直接肌肉注射到BALB/c小鼠体内，用ELISA检测血清中HCV C特异性抗体。通过³H胸腺嘧啶核苷掺入法测定实验小鼠和对照小鼠脾细胞的增殖反应，用标准的4小时⁵¹Cr释放试验检测细胞毒性T淋巴细胞（CTL）活性。
所有免疫小鼠均产生了抗HCV核心抗体，且该抗体呈剂量和免疫次数依赖性。免疫小鼠对HCV核心抗原的淋巴细胞增殖反应高于对照小鼠（P < 0.05），刺激指数（SI）为2.42 - 6.10。CTL可诱导约63.13%的pcDNA HCV C转染的SP2/0靶细胞裂解。
提示HCV C基因免疫是一种有前景的预防HCV感染的疫苗接种方法。
