Yang F, Zhang Y, McGee L
Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhonghua Yi Xue Za Zhi. 2001 May 25;81(10):589-92.
To investigate the population biology of Streptococcus pneumoniae carried by healthy children.
Drug resistant patterns and serotypes of 222 strains were determined. PFGE, BOX PCR and pbp fingerprint techniques were used to analyze the homology among strains.
Among 222 nasopharyngeal isolates from 5 day-care centers, there were 32 penicillin intermediate Streptococcus pneumoniae (PISP, MIC 0.125 approximately 1 mg/L), and no penicillin resistant Streptococcus pneumoniae (PRSP, MIC >/= 2 mg/L) was found. The main serotypes of all strains were 23F (25.7%), 6A (13.1%), 19F (10.8%), and 6B (8.1%). The serotypes of PISP were mainly 23F (50%), 19A (15.6%) and 19F (15.6%). Among the 32 PISP strains, 8 BOX PCR clusters and 7 PFGE types were identified. The resistance patterns, serotypes, BOX PCR and pbp fingerprints of PFGE type A strains were highly consistent. In day care center (DCC) SY, the penicillin resistant rate was 31.8%, being much higher than the average level. Twenty of the 21 PISP strains isolated from this DCC belonged to PFGE type A or B.
The penicillin resistant rate of Streptococcus pneumoniae carried by healthy children in 5 DCCs of Shanghai was still low. However in one DCC, there could be a prevalence of some resistant clones which resulted in high penicillin resistant rate.
研究健康儿童携带的肺炎链球菌的群体生物学特性。
测定222株菌株的耐药模式和血清型。采用脉冲场凝胶电泳（PFGE）、BOX-PCR和pbp指纹技术分析菌株间的同源性。
在来自5个日托中心的222株鼻咽分离株中，有32株青霉素中介肺炎链球菌（PISP，MIC为0.125～1mg/L），未发现青霉素耐药肺炎链球菌（PRSP，MIC≥2mg/L）。所有菌株的主要血清型为23F（25.7%）、6A（13.1%）、19F（10.8%）和6B（8.1%）。PISP的血清型主要为23F（50%）、19A（15.6%）和19F（15.6%）。在32株PISP菌株中，鉴定出8个BOX-PCR簇和7种PFGE型。PFGE A型菌株的耐药模式、血清型、BOX-PCR和pbp指纹高度一致。在日托中心SY，青霉素耐药率为31.8%，远高于平均水平。从该日托中心分离的21株PISP菌株中有20株属于PFGE A型或B型。
上海5个日托中心健康儿童携带的肺炎链球菌青霉素耐药率仍较低。然而，在一个日托中心，可能存在一些耐药克隆的流行，导致青霉素耐药率较高。
